bs-2075R [Primary Antibody]
Heme oxygenase 1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Heme Oxygenase 1, Heme Oxygenase 2

Immunogen Range: 101-200/288


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3162

Swiss Prot: P09601

Source: KLH conjugated synthetic peptide derived from human Heme Oxygenase 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Mouse
Rat
Pig
Chicken

Predicted Cross Reactive Species: Dog
Cow
Sheep
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wei, Bo, et al. "Regulation of antioxidant system, lipids and fatty acid β-oxidation contributes to the cardioprotective effect of sodium tanshinone IIA sulphonate in isoproterenol-induced myocardial infarction in rats."Atherosclerosis (2013).Read more>>
  • Dey, Mahua, et al. "Heme oxygenase-1 protects regulatory T cells from hypoxia-induced cellular stress in an experimental mouse brain tumor model." Journal of Neuroimmunology (2013).Read more>>
  • Song, Wei, et al. "Astroglial Heme Oxygenase-1 and the Origin of Corpora Amylacea in Aging and Degenerating Neural Tissues." Experimental Neurology (2014).Read more>>
  • Liu, Cui-Zhen, et al. "HBSP attenuates lipopolysaccharide-induced inflammatory response in human renal proximal tubular epithelial cells by induction of Nrf2 via PI3K/Akt pathway." Int J Clin Exp Med 10.1 (2017): 587-597.Read more>>
  • Zeng, X-P., et al. "Tert-Butylhydroquinone Protects Liver Against Ischemia/Reperfusion Injury in Rats Through Nrf2-Activating Anti-Oxidative Activity." Transplantation Proceedings. Vol. 49. , 2017.Read more>>
  • Wang, Yun, et al. "TBHQ Alleviated Endoplasmic Reticulum Stress-Apoptosis and Oxidative Stress by PERK-Nrf2 Crosstalk in Methamphetamine-Induced Chronic Pulmonary Toxicity." Oxidative Medicine and Cellular Longevity 2017 (2017).Read more>>
  • Wang, Yun, et al. "Fluoxetine protects against methamphetamine‑induced lung inflammation by suppressing oxidative stress through the SERT/p38 MAPK/Nrf2 pathway in rats." Molecular Medicine Reports 15.2 (2017): 673-680.Read more>>
  • Tao, Kai, Jinghai Chen, and Lingyun Wang. "Effects of berberine on the expressions of NRF2 and HO-1 in endothelial cells of diabetic rat." Biomedical Research 28.9 (2017): 3860-3864.Read more>>
  • Maïga, Souleymane, et al. "Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model." PLOS ONE 12.7 (2017): e0181067.Read more>>
  • Bai, Juan, et al. "Central administration of tert-butylhydroquinone attenuates hypertension via regulating Nrf2 signaling in the hypothalamic paraventricular nucleus of hypertensive rats." Toxicology and Applied Pharmacology (2017).Read more>>
  • Xuan et al. Astaxanthin blocks preeclampsia progression by suppressing oxidative stress and inflammation. (2016) Mol.Med.Rep. 14:2697-704Read more>>
  • Wei et al. The cardioprotective effect of sodium tanshinone IIA sulfonate and the optimizing of therapeutic time window in myocardial ischemia/reperfusion injury in rats. (2014) Atherosclerosis. 235:318-27Read more>>
  • Wang et al. Comparison of HMOX1 expression and enzyme activity in blue-shelled chickens and brown-shelled chickens. (2013) Genet.Mol.Biol. 36:282-6Read more>>
  • Ni et al. Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling. (2015) Int.J.Clin.Exp.Pathol. 8:7752-61Read more>>
  • Zhang et al. Protective effect of heme oxygenase-1 on Wistar rats with heart failure through the inhibition of inflammation and amelioration of intestinal microcirculation. (2015) J.Geriatr.Cardiol. 12:353-65Read more>>
  • Li J et al. Baicalin mitigated Mycoplasma gallisepticum-induced structural damage and attenuated oxidative stress and apoptosis in chicken thymus through the Nrf2/HO-1 defence pathway. Vet Res. 2019 Oct 21;50(1):83.Read more>>
  • Han C et al. Florfenicol induces oxidative stress and hepatocyte apoptosis in broilers via Nrf2 pathway. Ecotoxicol Environ Saf. 2020 Mar 15;191:110239.Read more>>
  • Giraud S et al. The inhibition of eIF5A hypusination by GC7, a preconditioning protocol to prevent brain death\induced renal injuries in a preclinical porcine kidney transplantation model. Am J Transplant. 2020 Dec;20(12):3326-3340.Read more>>
  • Zhang Yifanet al. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxid Med Cell Longev. 2020 Jul 9;2020:6428498.Read more>>
  • Jacopo Di Gregorio. et al. UBXN7 cofactor of CRL3KEAP1 and CRL2VHL ubiquitin ligase complexes mediates reciprocal regulation of NRF2 and HIF-1 proteins. Bba-Mol Cell Res. 2021 Apr;1868:118963Read more>>
  • Yingran Liang. et al. Crocin ameliorates arsenic trioxide?induced cardiotoxicity via Keap1-Nrf2/HO-1 pathway: Reducing oxidative stress, inflammation, and apoptosis. Biomed Pharmacother. 2020 Nov;131:110713Read more>>
  • L.X. Li. et al. MiR-23a-5p exacerbates intestinal ischemiaCreperfusion injury by promoting oxidative stress via targeting PPAR alpha. Biochem Pharmacol. 2020 Oct;180:114194Read more>>
  • Xiao Wang. et al. Florfenicol induces renal toxicity in chicks by promoting oxidative stress and apoptosis. Environ Sci Pollut R. 2021 Jan;28(1):936-946Read more>>
  • Mahmoud E. Youssef. et al. 7-nAChRs-mediated therapeutic angiogenesis accounts for the advantageous effect of low nicotine doses against myocardial infarction in rats. Eur J Pharmacol. 2021 Mar;:173996Read more>>
  • Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 ApRead more>>
  • Feng Gao. et al. Aucubin ameliorates the LPS\induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF\B and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27Read more>>
  • Xinrui Zhang. et al. Wan-Nian-Qing, a Herbal Composite Prescription, Suppresses the Progression of Liver Cancer in Mice by Regulating Immune Response. Front Oncol. 2021; 11: 696282Read more>>
  • Caijun Zhao. et al. Kynurenic acid protects against mastitis in mice by ameliorating inflammatory responses and enhancing blood-milk barrier integrity. Mol Immunol. 2021 Sep;137:134Read more>>
  • Zihui Wang. et al. Heat stress and hypoxia inhibit the secretion of androgens and induce epithelial-to-mesenchymal transition associated with activated TGF-_/Smad signaling in canine cryptorchidism. REPROD DOMEST ANIM. 2022 Jun 09Read more>>
  • Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297Read more>>
  • Yiyun Qian. et al. Seeds of Ginkgo biloba L. inhibit oxidative stress and inflammation induced by cigarette smoke in COPD rats through the Nrf2 pathway. J ETHNOPHARMACOL. 2023 Jan;301:115758Read more>>
  • Rui Sang. et al. Taraxasterol alleviates aflatoxin B1-induced liver damage in broiler chickens via regulation of oxidative stress, apoptosis and autophagy. ECOTOX ENVIRON SAFE. 2023 Feb;251:114546Read more>>
  • Huanhuan Wang. et al. The Pigments in Eggshell with Different Color and the Pigment Regulatory Genes Expression in Corresponding Chickens Shell Gland. ANIMAL. 2023 Mar;:100776Read more>>
  • Lei Liu. et al. Phenylboronic Ester-Bridged Chitosan/Myricetin Nanomicelle for Penetrating the Endothelial Barrier and Regulating Macrophage Polarization and Inflammation against Ischemic Diseases. ACS BIOMATER-SCI ENG. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Chen Chunni. et al. Study on the targeted regulation of Scutellaria baicalensis leaf on glutamineCglutamate metabolism and glutathione synthesis in the liver of D-gal ageing rats. J PHARM PHARMACOL. 2023 JuRead more>>
  • Yu-Sheng Shi. et al. Dendrobine rescues cognitive dysfunction in diabetic encephalopathy by inhibiting ferroptosis via activating Nrf2/GPX4 axis. PHYTOMEDICINE. 2023 Jul;:154993Read more>>
  • Qingyu Ding. et al. N-acetylcysteine alleviates oxidative stress and apoptosis and prevents skeletal muscle atrophy in type 1 diabetes mellitus through the NRF2/HO-1 pathway. LIFE SCI. 2023 Sep;329:121975Read more>>
  • Lei Liu. et al. Myricetin Oligomer Triggers Multi-Receptor Mediated Penetration and Autophagic Restoration of Blood-Brain Barrier for Ischemic Stroke Treatment. ACS NANO. 2024;XXXX(XXX):XXX-XXXRead more>>
  • Yao Wang. et al. Acetyl-11-keto-beta-boswellic acid modulates macrophage polarization and Schwann cell migration to accelerate spinal cord injury repair in rats. CNS NEUROSCI THER. 2024 Mar;30(3):e14642Read more>>
  • Jing Zhao. et al. Differentiation of intestinal stem cells toward goblet cells under systemic iron overload stress are associated with inhibition of Notch signaling pathway and ferroptosis. REDOX BIOL. 2024 Jun;72:10316Read more>>
  • Wen E. et al. The mitochondria-targeted Kaempferol nanoparticle ameliorates severe acute pancreatitis. J NANOBIOTECHNOL. 2024 Dec;22(1):1-15Read more>>
  • Xu-rui Gu. et al. Layer-by-Layer Assembly of Renal-Targeted Polymeric Nanoparticles for Robust Arginase-2 Knockdown and Contrast-Induced Acute Kidney Injury Prevention. ADV HEALTHC MATER. 2024 May;:2304675Read more>>
  • Hong Jin. et al. Unlocking the Therapeutic Potential of a Manila Clam-Derived Antioxidant Peptide: Insights into Mechanisms of Action and Cytoprotective Effects against Oxidative Stress. FOODS. 2024 Jan;13(8):116Read more>>
  • Yu Zhang. et al. Follicular fluid-derived exosomal HMOX1 promotes granulosa cell ferroptosis involved in follicular atresia in geese (Anser cygnoides). POULTRY SCI. 2024 May;:103912Read more>>
  • Liang Wang. et al. Myrislignan ameliorates the progression of osteoarthritis: An in vitro and in vivo study. INT IMMUNOPHARMACOL. 2024 Oct;140:112887Read more>>
  • Sneha Kumari. et al. Understanding the molecular basis of anti-fibrotic potential of intranasal curcumin and its association with mitochondrial homeostasis in silica-exposed mice. MITOCHONDRION. 2024 Sep;78:101943Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining


293T cell lysate probed (bs-2075R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT. \n


Lane 1: Human HepG2 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human DU145 cell lysates; Lane 4: Human H9C2 cell lysates; Lane 5: Human 293T cell lysates probed with Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.