bs-2073R-Cy3 [Conjugated Primary Antibody]
GSK3 Alpha/Beta(Tyr279+Tyr216) Antibody, Cy3 Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: GSK3 Alpha/Beta Tyr279+Tyr216

Modification Site: Tyr279+Tyr216

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2931

Swiss Prot: P49840

Source: KLH conjugated synthetic phosphopeptide derived from human GSK3 Alpha/Beta around the phosphorylation site of Tyr279/Tyr216

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 47/51


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Hai-hua, et al. "Involvement of GSK3 and PP2A in ginsenoside Rb1's attenuation of aluminum-induced tau hyperphosphorylation." Behavioural Brain Research (2012).Read more>>
  • J Song. et al. MSCs reduce airway remodeling in the lungs of asthmatic rats through the Wnt/-catenin signaling pathway.. Eur Rev Med Pharmaco. 2020 Nov;24(21):11199-11211Read more>>
  • Cheng, Baixiang. et al. Distinctive Roles of Wnt Signaling in Chondrogenic Differentiation of BMSCs under Coupling of Pressure and Platelet-Rich Fibrin. Tissue Engineering and Regenerative Medicine. 2022 Apr;:1-15Read more>>
  • Cuicui Zhuang. et al. Escherichia coli infection induces ferroptosis in bovine mammary epithelial cells by activating the Wnt/-catenin pathway-mediated mitophagy. MITOCHONDRION. 2024 Sep;78:101921Read more>>
VALIDATION IMAGES