Host: Rabbit
Target Protein: FAK
Immunogen Range: 131-230/1052
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7424
Swiss Prot: Q05397
Source: KLH conjugated synthetic peptide derived from human FAK
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 116
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
- Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 MaRead more>>
- Wenzhao Wang. et al. 3D printing of personalized magnesium composite bone tissue engineering scaffold for bone and angiogenesis regeneration. CHEM ENG J. 2024 Mar;484:149444Read more>>
- Dating Pei. et al. Modulation of macrophage polarization by secondary cross-linked hyaluronan-dopamine hydrogels. INT J BIOL MACROMOL. 2024 Jun;270:132417Read more>>
- Xin Bin. et al. circMTO1/miR-30c-5p/SOCS3 axis alleviates oral submucous fibrosis through inhibiting fibroblastmyofibroblast transition. J ORAL PATHOL MED. 2024 MayRead more>>
- Chenlei Li. et al. Plexin D1 Negatively Regulates Macrophage-derived Foam Cell Migration via the Focal Adhesion Kinase/Paxillin Pathway. BIOCHEM BIOPH RES CO. 2024 Jun;:150236Read more>>
Lane 1: Mouse Testis lysates; Lane 2: Mouse NIH/3T3 cell lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse Testis lysates; Lane 5: Human 293T cell lysates; Lane 6: Human A431 cell lysates; Lane 7: Human HUVEC cell lysates; Lane 8: Human MDA-MB-231 cell lysates; Lane 9: Human A549 cell lysates probed with FAK Polyclonal Antibody, Unconjugated (bs-20735R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAK Polyclonal Antibody, Unconjugated (bs-20735R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAK Polyclonal Antibody, Unconjugated (bs-20735R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAK Polyclonal Antibody, Unconjugated (bs-20735R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAK Polyclonal Antibody, Unconjugated (bs-20735R) at 1:200 overnight at 4°C, DAB staining.