bs-20730R [Primary Antibody]
Dopamine D2 Receptor, Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Dopamine D2 Receptor,

Immunogen Range: 301-400/443


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1813

Swiss Prot: P14416

Source: KLH conjugated synthetic peptide derived from human Dopamine D2 Receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

7 transmembrane receptor (rhodopsin family). This family contains, amongst other G-protein-coupled receptors (GCPRs), members of the opsin family, which have been considered to be typical members of the rhodopsin superfamily. They share several motifs, mainly the seven transmembrane helices, GCPRs of the rhodopsin superfamily. All opsins bind a chromophore, such as 11-cis-retinal. The function of most opsins other than the photoisomerases is split into two steps: light absorption and G-protein activation. Photoisomerases, on the other hand, are not coupled to G-proteins - they are thought to generate and supply the chromophore that is used by visual opsins.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 51


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang YH et al. Isosibiricin inhibits microglial activation by targeting the dopamine D1/D2 receptor-dependent NLRP3/caspase-1 inflammasome pathway. Acta Pharmacol Sin. 2019 Sep 10. Read more>>
  • Xuezhi Zhang. et al. Natural emodin reduces myocardial ischemia/reperfusion injury by modulating the RUNX1/miR?142?3p/DRD2 pathway and attenuating inflammation. EXP THER MED. 2022 Dec;24(6):1-11Read more>>
  • Xiaoyan Zheng. et al. Novel findings from arsenic?lead combined exposure in mouse testicular TM4 Sertoli cells based on transcriptomics. SCI TOTAL ENVIRON. 2023 Dec;:169611Read more>>
VALIDATION IMAGES

Mouse eye lysates probed with Dopamine D2 Receptor Polyclonal Antibody, Unconjugated (bs-20730R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse BV-2 cell lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Rat RSC96 cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human U-87MG cell lysates probed with Dopamine D2 Receptor Polyclonal Antibody, Unconjugated (bs-20730R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


25 ug total protein per lane of various lysates (see on figure) probed with DRD2 polyclonal antibody, unconjugated (bs-20730R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.