Host: Rabbit
Target Protein: Galectin 3
Immunogen Range: 181-250/250
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1385
Swiss Prot: P17931
Source: KLH conjugated synthetic peptide derived from human Galectin 3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 29
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
For research use only. Not intended for diagnostic or therapeutic use.
- Wang H et al. The effect of exposure time and concentration of airborne PM2. 5 on lung injury in mice: A transcriptome analysis. Redox Biol. 2019 Jul 2;26:101264. Read more>>
- Gao J et al. Metformin protects against PM2. 5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2. Redox Biol. 2019 Oct 19;28:101345.Read more>>
- Yongcan Wu. et al. Probiotics ameliorates pulmonary inflammation via modulating gut microbiota and rectifying Th17/Treg imbalance in a rat model of PM2.5 induced lung injury. ECOTOX ENVIRON SAFE. 2022 Oct;244:11406Read more>>
- aoxin Qiao. et al. Curcumin attenuates AFB1-induced duck liver injury by inhibiting oxidative stress and lysosomal damage. FOOD CHEM TOXICOL. 2022 Dec;:113593Read more>>
Lane 1: Human Siha cell lysates; Lane 2: Human U-87MG cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human LOVO cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human Hela cell lysates probed with Galectin 3 Polyclonal Antibody, Unconjugated (bs-20700R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Galectin 3 Antibody(bs-20700R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).