bs-20694R-Cy5.5 [Conjugated Primary Antibody]
Beta tubulin Polyclonal Antibody, Cy5.5 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Beta tubulin

Immunogen Range: 61-160/444


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 203068

Swiss Prot: P07437

Source: KLH conjugated synthetic peptide derived from human Beta tubulin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.

Conjugation: Cy5.5

Excitation/ Emission: 675nm/694nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 55


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhen Zhang. et al. Tanshinone IIA regulates fibroblast proliferation and migration and post-surgery arthrofibrosis through the autophagy-mediated PI3K and AMPK-mTOR signaling pathway. Am J Transl Res. 2021; 13(2): 565C584Read more>>
  • Li, Jiao. et al. Adaptation of AMPK-mTOR-signal pathways and lipid metabolism in response to low- and high-level rapeseed meal diet in Chinese perch (Siniperca chuatsi). J Comp Physiol B. 2021 Sep;191(5):881-894Read more>>
  • Shi, Linjie. et al. Memory regulation in feeding habit transformation to dead prey fish of Chinese perch (Siniperca chuatsi). 2021 Sep 28Read more>>
  • Chen, Lei. et al. SARS-CoV-2 nucleocapsid protein triggers hyperinflammation via protein-protein interaction-mediated intracellular Cl_ accumulation in respiratory epithelium. SIGNAL TRANSDUCT TAR. 2022 Jul;7(1):1-13Read more>>
  • Wang Yuan. et al. Effect of one-time high load exercise on skeletal muscle injury in rats of different genders: oxidative stress and mitochondrial responses. ACTA CIR BRAS. 2022 Dec;37Read more>>
  • Wang Kaiyuan. et al. Gas therapy potentiates aggregation-induced emission luminogen-based photoimmunotherapy of poorly immunogenic tumors through cGAS-STING pathway activation. NAT COMMUN. 2023 May;14(1):1-19Read more>>
  • Fang Chen. et al. Disordered GPR43/NLRP3 expression in peripheral leukocytes of patients with atrial fibrillation is associated with intestinal short chain fatty acids levels. EUR J MED RES. 2024 Dec;29(1):1-13Read more>>
VALIDATION IMAGES