bs-2066R
[Primary Antibody]
GSK-3 Beta(Ser9) Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: GSK-3 Beta Ser9
Modification Site: Ser9
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2932
Swiss Prot: P49841
Source: KLH conjugated synthetic phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser9
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 47
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Mikami, Norihisa, et al. Read more>>
- Li, Zhao, et al. "The aqueous extract of Curcuma wenyujin rescues learning and memory deficits through PI3k/Akt/GSK-3β pathway in Aβ-induced AD mice." Biomedical Research 28.17 (2017): 7438-7442.Read more>>
- Zhang et al. Over-Expressed Twist Associates with Markers of Epithelial Mesenchymal Transition and Predicts Poor Prognosis in Breast Cancers via ERK and Akt Activation. (2015) PLoS.One. 10:e0135851Read more>>
- Wang Y et al. High Concentration of Aspirin Induces Apoptosis in Rat Tendon Stem Cells via Inhibition of the Wnt/β-Catenin Pathway. (2018) Cell Physiol Biochem;50(6):2046-2059.Read more>>
- Ren et al. GSK-3β inhibits autophagy and enhances radiosensitivity in non-small cell lung cancer. (2018) Diagn.Pathol. 13:33Read more>>
- Sun L et al. MiR-26a promotes fracture healing of nonunion rats possibly by targeting SOSTDC1 and further activating Wnt/β-catenin signaling pathway. Mol Cell Biochem. 2019 Jul 16.Read more>>
- Tong Xu. et al. Lithium chloride represses abdominal aortic aneurysm via regulating GSK3/SIRT1/NF-B signaling pathway. Free Radical Bio Med. 2021 Apr;166:1Read more>>
- Peiqi Zhu. et al. Panax notoginseng saponins promote endothelial progenitor cell angiogenesis via the Wnt/-catenin pathway. Bmc Complem Altern M. 2021 Dec;21(1):1-11Read more>>
- Qingxin Fan. et al. Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/-Catenin Signaling in 3T3-L1 Adipocytes. Med Sci Monitor. 2021; 27: e928619-1Ce928619-1Read more>>
- Junying Lan. et al. Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model. Neuropharmacology. 2021 Sep;196:108689Read more>>
- Ling Xie. et al. Suppression of GOLM1 by EGCG through HGF/HGFR/AKT/GSK-3/-catenin/c-Myc signaling pathway inhibits cell migration of MDA-MB-231. Food Chem Toxicol. 2021 Nov;157:112574Read more>>
- Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
- Wang, Lili. et al. Progranulin improves neural development via the PI3K/Akt/GSK-3_ pathway in the cerebellum of a VPA-induced rat model of ASD. Transl Psychiat. 2022 Mar;12(1):1-14Read more>>
- Huimin Wang. et al. Chronic exposure of bisphenol-A impairs cognitive function and disrupts hippocampal insulin signaling pathway in male mice. TOXICOLOGY. 2022 Apr;472:153192Read more>>
- Yang, Yuying. et al. FAT4 activation inhibits epithelial-mesenchymal transition (EMT) by promoting autophagy in H2228/Cer cells. MED ONCOL. 2023 Jan;40(1):1-12Read more>>
- Jiang Hongyuan. et al. Establishment of a prognostic risk model for osteosarcoma and mechanistic investigation. FRONT PHARMACOL. 2024 Apr;15Read more>>
- Wang Jianan. et al. Association of attenuated leptin signaling pathways with impaired cardiac function under prolonged high-altitude hypoxia. SCI REP-UK. 2024 May;14(1):1-18Read more>>
VALIDATION IMAGES
Lane 1: rat testis lysates probed with Rabbit Anti-GSK-3 Beta(Ser9) Polyclonal Antibody, Unconjugated (bs-2066R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-GSK-3 Beta (Ser9)) polyclonal Antibody, Unconjugated (bs-2066R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL2 Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).