bs-20658R [Primary Antibody]
FSH Receptor Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FSH Receptor

Immunogen Range: 21-100/695


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2492

Swiss Prot: P23945

Source: KLH conjugated synthetic peptide derived from human FSH receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Induces cAMP production through the activation of PI3K-AKT and SRC-ERK1/2 signaling pathways.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 78


Cross Reactive Species: Human
Mouse
Rat
Others

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Junjie, et al. "Seasonal expression of luteinizing hormone receptor and follicle stimulating hormone receptor in testes of the wild ground squirrels (Citellus dauricus Brandt)." Acta Histochemica (2017).Read more>>
  • Yang Z et al. A pilot study on polycystic ovarian syndrome caused by neonatal exposure to tributyltin and bisphenol A in rats. Chemosphere. 2019 Sep;231:151-160. Read more>>
  • Zhou Z et al. Maternal high-fat diet impairs follicular development of offspring through intraovarian kisspeptin/GPR54 system. Reprod Biol Endocrinol. 2019 Jan 22;17(1):13. Read more>>
  • Wang Q et al. Heat shock pretreatment improves mesenchymal stem cell viability by heat shock proteins and autophagy to prevent cisplatin-induced granulosa cell apoptosis. Stem Cell Res Ther. 2019 Nov 26;10(1):348.Read more>>
  • Xiyan Wang. et al. The optimized research of the in vitro culture of preantral follicles in mice. J Clin Lab Anal. 2020 Nov;34(11):e23498Read more>>
  • Nikol Dibus. et al. FBXO38 Ubiquitin Ligase Controls Sertoli Cell Maturation. FRONT CELL DEV BIOL. 2022; 10: 914053Read more>>
  • Jianfang Huang. et al. Kallistatin prevents ovarian hyperstimulation syndrome by regulating vascular leakage. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2022 JulRead more>>
  • Luo, Qianqian. et al. hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms. REPROD BIOL ENDOCRIN. 2022 Dec;20(1):1-14Read more>>
  • Jana Petrusov. et al. Two complementary approaches for efficient isolation of Sertoli cells for transcriptomic analysis. FRONT CELL DEV BIOL. 2022; 10: 972017Read more>>
  • Sijie Fan. et al. Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (Citellus dauricus Brandt). INT J MOL SCI. 2022 Jan;23(23):14698Read more>>
  • Qingjing Gao. et al. Seasonal changes in endoplasmic reticulum stress and steroidogenesis in the ovary of the wild ground squirrels (Citellus dauricus Brandt). GEN COMP ENDOCR. 2023 Nov;343:114368Read more>>
  • Polat Blent. et al. The effects of low-level laser therapy on polycystic ovarian syndrome in rats: three different dosages. LASER MED SCI. 2023 Dec;38(1):1-7Read more>>
VALIDATION IMAGES

Mouse ovary lysates probed with FSH Receptor Polyclonal Antibody (bs-20658R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FSH Receptor Polyclonal Antibody at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed bysecondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.