bs-2060R [Primary Antibody]
AQP9 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AQP9

Immunogen Range: 201-295/295


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 366

Swiss Prot: O43315

Source: KLH conjugated synthetic peptide derived from human AQP9

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 32


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Cow
Sheep
Pig
Rabbit
GuineaPig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chau, David, et al. "Azacytidine sensitizes acute myeloid leukemia cells to arsenic trioxide by up-regulating the arsenic transporter aquaglyceroporin 9."Journal of Hematology & Oncology 8.1 (2015): 46.Read more>>
  • DAVID, CHAU HAU WING. "POTENTIAL THERAPEUTIC APPLICATION OF ARSENIC TRIOXIDE IN ACUTE MYELOID LEUKAEMIA."Read more>>
  • DAVID, CHAU HAU WING. "POTENTIAL THERAPEUTIC APPLICATION OF ARSENIC TRIOXIDE IN ACUTE MYELOID LEUKAEMIA."Read more>>
  • Vicente‐Carrillo, A., et al. "Membrane Stress During Thawing Elicits Redistribution of Aquaporin 7 But Not of Aquaporin 9 in Boar Spermatozoa."Reproduction in Domestic Animals (2016).Read more>>
  • Sato K et al. Different Prognostic Implications of Aquaporin-1 and Aquaporin-5 Expression among Different Histological Types of Ovarian Carcinoma.Pathol Oncol Res. 2018 Jul 19. Read more>>
  • Ji Woo Im. et al. Stichoposide C and Rhizochalin as Potential Aquaglyceroporin Modulators. MAR DRUGS. 2024 Aug;22(8):335Read more>>
VALIDATION IMAGES

Lane 1: Mouse intestine lysates probed with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Mouse liver lysates probed with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.


Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-AQP9 Polyclonal Antibody, Unconjugated (bs-2060R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


A549 probed with AQP9 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-2060R-A647) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).