bs-20594R-Cy7 [Conjugated Primary Antibody]
TLR4 Polyclonal Antibody, Cy7 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TLR4

Immunogen Range: 701-800/839


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7099

Swiss Prot: O00206

Source: KLH conjugated synthetic peptide derived from human TLR4

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:9237759, PubMed:10835634). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+ require non-conserved histidines and are, therefore, species-specific (PubMed:20711192). Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression (PubMed:15809303). In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187).

Conjugation: Cy7

Excitation/ Emission: 743nm/767nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 90


Cross Reactive Species: Human
Mouse
Rat
Cow

Predicted Cross Reactive Species: Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Dong W et al. Polychlorinated biphenyl quinone induces caspase 1-mediated pyroptosis through the induction of pro-inflammatory HMGB1-TLR4-NLRP3-GSDMD signal axis. Chem Res Toxicol. 2019 Apr 23. Read more>>
  • Yuan FH et al. microRNA‐30a inhibits the liver cell proliferation and promotes cell apoptosis through the JAK/STAT signaling pathway by targeting SOCS‐1 in rats with sepsis. J Cell Physiol. 2019 Apr 10. Read more>>
  • Liu W et al. Suppressive effect of glycyrrhizic acid against lipopolysaccharide-induced neuroinflammation and cognitive impairment in C57 mice via toll-like receptor 4 signaling pathway. Food Nutr Res. 2019 Apr 29;63.Read more>>
  • Hu N et al. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway. J Ethnopharmacol. 2019 Nov 1:112361.Read more>>
  • Li X et al. Leptin improves intestinal flora dysfunction in mice with high-fat diet-induced obesity. J Int Med Res. 2020 Jun;48(6):300060520920062.Read more>>
  • Wenjuan Chenet al. Integrated analysis of a lncRNA?mRNA network reveals a potential mechanism underlying necrotizing enterocolitis. Mol Med Rep. 2020 Jul;22(1):423-435.Read more>>
  • Zhao Yonglin. et al. Inhibition of Macrophage Migration Inhibitory Factor Protects against Inflammation through a Toll-like Receptor-Related Pathway after Diffuse Axonal Injury in Rats. Biomed Res Int. 2020;2020:5946205Read more>>
  • Dongfeng Cai. et al. The Effects of microRNA-515-5p on the Toll-Like Receptor 4 (TLR4)/JNK Signaling Pathway and WNT1-Inducible-Signaling Pathway Protein 1 (WISP-1) Expression in Rheumatoid Arthritis Fibroblast-Like Synovial (RAFLS) Cells Following Treatment with Receptor Act. Med Sci Monitor. 2020; 26: e920611-1Ce920611-9Read more>>
  • Karadag Remzi. et al. Effects of Different Doses of Systemic Isotretinoin on Eyes: A Histopathological and Immunohistochemical Study in Rats. Cornea. 2020 May;39(5):621-627Read more>>
  • Naihua Hu. et al. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-B and TGF-/smads signaling pathways. J Ethnopharmacol. 2020 Nov;262:113275Read more>>
  • Sijie Yuan. et al. Bacteroides vulgatus diminishes colonic microbiota dysbiosis ameliorating lumbar bone loss in ovariectomized mice. Bone. 2021 Jan;142:11571Read more>>
  • Xuelian Ma. et al. Down-regulated long non-coding RNA RMST ameliorates dopaminergic neuron damage in Parkinsons disease rats via regulation of TLR/NF-B signaling pathway. Brain Res Bull. 2021 ApRead more>>
  • Zhao Xueqin. et al. The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier. Front Microbiol. 2021 Jun;12:1392Read more>>
  • Li Liu. et al. Inhibition of inducible nitric oxide synthase improved erectile dysfunction in rats with type 1 diabetes. 2021 Jun 16Read more>>
  • Jiali Yang. et al. Baicalin Rescues Cognitive Dysfunction, Mitigates Neurodegeneration, and Exerts Anti-Epileptic Effects Through Activating TLR4/MYD88/Caspase-3 Pathway in Rats. Drug Des Dev Ther. 2021 Jul;15:3163-318Read more>>
  • Yitong Pan. et al. N-acetyl-L-tryptophan attenuates hepatic ischemia-reperfusion injury via regulating TLR4/NLRP3 signaling pathway in rats. Peerj. 2021 Aug;9:e11909Read more>>
  • Alessandro Del Re. et al. Ultramicronized Palmitoylethanolamide Inhibits NLRP3 Inflammasome Expression and Pro-Inflammatory Response Activated by SARS-CoV-2 Spike Protein in Cultured Murine Alveolar Macrophages. Metabolites. 2021 Sep;11(9):592Read more>>
  • Meilan Xue. et al. Neuroprotective effect of fucoidan by regulating gut-microbiota-brain axis in alcohol withdrawal mice. J Funct Foods. 2021 Nov;86:104726Read more>>
  • Chiara Corpetti. et al. Cannabidiol inhibits SARS-Cov-2 spike (S) protein-induced cytotoxicity and inflammation through a PPAR-dependent TLR4/NLRP3/Caspase-1 signaling suppression in Caco-2 cell line. 2021 Oct 12Read more>>
  • Shi-Qing Cai. et al. The In Vitro Anti-Inflammatory Activities of Galangin and Quercetin towards the LPS-Injured Rat Intestinal Epithelial (IEC-6) Cells as Affected by Heat Treatment. Molecules. 2021 Jan;26(24):7495Read more>>
  • Xu X. et al. <i>Alhagi pseudalhagi</i> Extract Exerts Protective Effects Against Intestinal Inflammation in Ulcerative Colitis by Affecting TLR<sub>4</sub>-Dependent NF-B Signaling Pathways.. Front Pharmacol. 2021 Nov;12:764602-764602Read more>>
  • Zou Xiong. et al. Toll-Like Receptors Serve as Biomarkers for Early Diagnosis and Prognosis Assessment of Kidney Renal Clear Cell Carcinoma by Influencing the Immune Microenvironment: Comprehensive Bioinformatics Analysis Combined With Experimental Validation. Front Mol Biosci. 2022 Jan;0:24Read more>>
  • Quan Rao. et al. Dendritic cell combination therapy reduces the toxicity of triptolide and ameliorates colitis in murine models. Drug Deliv. 2022;29(1):679-691Read more>>
  • Na Chen. et al. Casein Oligochitosan-Glycation by Transglutaminase Enhances the Anti-Inflammatory Potential of Casein Hydrolysates to the Lipopolysaccharide-Stimulated IEC-6 Cells. Nutrients. 2022 Jan;14(3):686Read more>>
  • Qi Wang. et al. Effect of oral administration of Limosilactobacillus reuteri on intestinal barrier function and mucosal immunity of suckling piglets. Ital J Anim Sci. 2022;21(1):612-623Read more>>
  • Shangren Wang. et al. A study of diabetes-induced erectile dysfunction treated with human umbilical cord mesenchymal stem cells. ANDROLOGIA. 2022 Apr 12Read more>>
  • Ziwei Lin. et al. Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo. INT J MOL SCI. 2022 Jan;23(14):7694Read more>>
  • Jiaojiao Wu. et al. The involvement of Pellino-1 downregulation in the modulation of visceral hypersensitivity via the TLR4/NF-_B pathway in the rat fastigial nucleus. NEUROSCI LETT. 2022 Sep;787:136815Read more>>
  • Ming Dong Si. et al. Swertia mussotii prevents high-fat diet-induced non-alcoholic fatty liver disease in rats by inhibiting expression the TLR4/MyD88 and the phosphorylation of NF-B. PHARM BIOL. 2022 Oct 07Read more>>
  • Na Chen. et al. Casein Lactose-Glycation of the Maillard-Type Attenuates the Anti-Inflammatory Potential of Casein Hydrolysate to IEC-6 Cells with Lipopolysaccharide Stimulation. NUTRIENTS. 2022 Jan;14(23):5067Read more>>
  • Chaojie Chen. et al. Ganoderma lucidum polysaccharide inhibits HSC activation and liver fibrosis via targeting inflammation, apoptosis, cell cycle, and ECM-receptor interaction mediated by TGF-/Smad signaling. PHYTOMEDICINE. 2022 Dec;:154626Read more>>
  • Dongming Chen. et al. Study on the mechanism of Phellinus igniarius total flavonoids in reducing uric acid and protecting uric acid renal injury in vitro. HELIYON. 2023 Jan;9:e12979Read more>>
  • Yuanyuan Xing. et al. Artemisia ordosica polysaccharide ameliorated LPS-induced growth inhibition and intestinal injury in broilers through enhancing immune-regulation and antioxidant capacity. J NUTR BIOCHEM. 2023 Feb;:109284Read more>>
  • Wenfeng Hu. et al. Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects. BIOMEDICINES. 2023 Feb;11(2):463Read more>>
  • Schary Yeshai. et al. CRISPR-Cas9 editing of TLR4 to improve the outcome of cardiac cell therapy. SCI REP-UK. 2023 Mar;13(1):1-16Read more>>
  • Xiaoyin Zhang. et al. Proinflammatory S100A9 stimulates TLR4/NF-B signaling pathways causing enhanced phagocytic capacity of microglial cells. IMMUNOL LETT. 2023 Mar;255:54Read more>>
  • Li Chengnan. et al. Neutrophil-inspired photothermo-responsive drug delivery system for targeted treatment of bacterial infection and endotoxins neutralization. Biomaterials Research. 2023 Dec;27(1):1-16Read more>>
  • Yang Jun-Pu. et al. Glycyrrhizin ameliorates impaired glucose metabolism and ovarian dysfunction in a polycystic ovary syndrome mouse model. BIOL REPROD. 2023 ApRead more>>
  • Qian Zhang. et al. Shiwei Qingwen decoction regulates TLR4/NF-B signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide-induced acute lung injury. J ETHNOPHARMACOL. 2023 Sep;313:116615Read more>>
  • Huizhen Ma. et al. Bioactive NIR-II gold clusters for three-dimensional imaging and acute inflammation inhibition. SCI ADV. 2023 Aug;9(31)Read more>>
  • Antonia Otoo. et al. Emodin improves glucose metabolism and ovarian function in PCOS mice via the HMGB1/TLR4/NF-?B molecular pathway. REPRODUCTION. 2023 Aug;-1(aop)Read more>>
  • Yaoxing Li. et al. Echinacea purpurea (L.) Moench Polysaccharide Alleviates DSS-Induced Colitis in Rats by Restoring Th17/Treg Balance and Regulating Intestinal Flora. FOODS. 2023 Jan;12(23):4265Read more>>
  • Haifei Lu. et al. Neuroprotective effects of Shenghui decoction via inhibition of the JNK/p38 MAPK signaling pathway in an AlCl3-induced zebrafish (Danio rerio) model of Alzheimer's disease. J ETHNOPHARMACOL. 2024 Feb;:117993Read more>>
  • Giovanni Sarnelli. et al. Intranasal administration of Escherichia coli Nissle expressing the spike protein of SARS-CoV-2 induces long-term immunization and prevents spike protein-mediated lung injury in mice. BIOMED PHARMACOTHER. 2024 May;174:116441Read more>>
  • Ziheng Zhu. et al. Exploration of the molecular mechanism guiding Xinfeng capsule regulatory mechanism for rheumatoid arthritis inflammation. AM J TRANSL RES. 2024; 16(3): 973C987Read more>>
  • Feng Gao. et al. Goat milk exosomal microRNAs alleviate LPS-induced intestinal inflammation in mice. INT J BIOL MACROMOL. 2024 May;268:131698Read more>>
  • Hai-Xiang Guo. et al. Turtle peptide and its derivative peptide ameliorated DSS-induced ulcerative colitis by inhibiting inflammation and modulating the composition of the gut microbiota. INT IMMUNOPHARMACOL. 2024 May;132:112024Read more>>
  • Mao Yu-Zhi. et al. Pudilan Anti-inflammatory Oral Liquid and Organic Acid Component from Taraxaci Herba Attenuate Allergic Asthma in Young Mice through Toll-like Receptor 2/Toll-like Receptor 4 Signaling Pathway. World Journal of Traditional Chinese Medicine. 2024 Apr;:10.4103/wjtcm.wjtcm_64_24Read more>>
  • Dan Zhao. et al. Copper exposure induces inflammation and PANoptosis through the TLR4/NF-B signaling pathway, leading to testicular damage and impaired spermatogenesis in Wilson disease. CHEM-BIOL INTERACT. 2024 Jun;396:11106Read more>>
  • Jing Lian. et al. Acteoside and isoacteoside alleviate renal dysfunction and inflammation in lipopolysaccharide-induced acute kidney injuries through inhibition of NF-B signaling pathway. PLOS ONE. 2024 May;19(5):e030374Read more>>
VALIDATION IMAGES