bs-2044R
[Primary Antibody]
LOX 1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: LOX 1
Immunogen Range: 201-273/273
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 100009322
Swiss Prot: Q9XTA8
Source: KLH conjugated synthetic peptide derived from Rabbit LOX-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria (By similarity).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 31/50
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species: Cow
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Deng, Zu Yue, et al. "Resveratrol alleviates vascular inflammatory injury by inhibiting inflammasome activation in rats with hypercholesterolemia and vitamin D2 treatment." Inflammation Research: 1-12.Read more>>
- Li, Cui, et al. "The Role of LOX-1 in Innate Immunity to Aspergillus fumigatus in Corneal Epithelial CellsLOX-1 in Corneal Epithelium to Fungal Infection." Investigative Ophthalmology & Visual Science 56.6 (2015): 3593-3603.Read more>>
- Gao, Xinran, et al. "LOX-1 and TLR4 affect each other and regulate the generation of ROS in A. fumigatus keratitis." International Immunopharmacology 40 (2016): 392-399.Read more>>
- Shibata, Masa-Aki, et al. "Pathological and molecular analyses of atherosclerotic lesions in ApoE-knockout mice." Medical Molecular Morphology: 1-15.Read more>>
- Song Q et al. Potential mechanisms underlying the protective effects of salvianic acid A against atherosclerosis in vivo and vitro. (2019)Biomedicine & Pharmacotherapy, 109, 945–956. Read more>>
- Yang B et al. Polychlorinated Biphenyl Quinone Promotes Macrophage-Derived Foam Cells Formation. Chem Res Toxicol. 2019 Nov 13. Read more>>
- Gao X et al. Inhibition of LOX-1 prevents inflammation and photoreceptor cell death in retinal degeneration. Int Immunopharmacol. 2020 Mar;80:106190. Read more>>
- Qian Liet al. LOX-1 Regulates P. gingivalis-Induced Monocyte Migration and Adhesion to Human Umbilical Vein Endothelial Cells. Front Cell Dev Biol. 2020 Jul 14;8:596.Read more>>
- Sato, Kayoko. et al. Oxidative stress-responsive apoptosis-inducing protein in patients with heterozygous familial hypercholesterolemia. 2021 Jul 26Read more>>
- Tingting Hao. et al. Phosphatidylethanolamine alleviates OX-LDL-induced macrophage inflammation by upregulating autophagy and inhibiting NLRP1 inflammasome activation. FREE RADICAL BIO MED. 2023 Nov;208:402Read more>>
VALIDATION IMAGES
This image was generously provided by Zu Yue Deng from Zhejiang University of Technology. Human heart tissue probed with Rabbit Anti-LOX 1 Polyclonal Antibody, Unconjugated (bs-2044R). Protein bands were developed using an HRP system.
Formalin-fixed and paraffin embedded rat aorta labeled with Anti-LOX-1 Polyclonal Antibody, Unconjugated (bs-2044R) followed by conjugation to the secondary antibody and DAB staining
RSC96 cells probed with LOX 1 Polyclonal Antibody, Unconjugated (bs-2044R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human A549 cells probed with Rabbit Anti-LOX1 Polyclonal Antibody, Unconjugated (bs-2044R) (green) at 1:20 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LOX 1 Polyclonal Antibody, Unconjugated (bs-2044R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rabbit brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LOX 1 Polyclonal Antibody, Unconjugated (bs-2044R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Mouse lung lysates; Lane 2: Mouse cerebrum lysates; probed with LOX 1 Polyclonal Antibody, unconjugated (bs-2044R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Rabbit brain lysates; probed with LOX 1 Polyclonal Antibody, unconjugated (bs-2044R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Thp-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with LOX 1 Polyclonal Antibody(bs- 2044R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).