bs-20399R
[Primary Antibody]
HIF-1 Alpha Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: HIF-1 Alpha
Immunogen Range: 661-760/826
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3091
Swiss Prot: Q16665
Source: KLH conjugated synthetic peptide derived from human HIF-1 Alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 92
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Jian-xia Wen. et al. Therapeutic Effects and Potential Mechanism of Dehydroevodiamine on N-Methyl-N-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis. Phytomedicine. 2021 Jun;:153619Read more>>
- Yu-Fei Zhang. et al. Therapeutic effect of a traditional Chinese medicine formulation on experimental choroidal neovascularization in mouse. Int J Ophthalmol-Chi. 2021; 14(10): 1492C15Read more>>
- Yanmei Ding. et al. Taurine is an effective therapy against thiram induced tibial dyschondroplasia via HIF-1/VEGFA and -catenin/ GSK-3 pathways in broilers. Ecotox Environ Safe. 2021 Dec;228:112981Read more>>
- Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635Read more>>
- Feng Zhao. et al. Mechanism Repositioning Based on Integrative Pharmacology: Anti-Inflammatory Effect of Safflower in Myocardial Ischemia–Reperfusion Injury. INT J MOL SCI. 2023 Jan;24(6):5313Read more>>
- ingtan Kong. et al. Histopathological Growth Pattern Evolution of Tumor in VX2 Liver Cancer Model. PATHOL RES PRACT. 2023 Mar;:154401Read more>>
- Run-Feng Miao. et al. LncRNA CDKN2B-AS1 interacts with LIN28B to exacerbate sepsis-induced acute lung injury by inducing HIF-1/NLRP3-mediated pyroptosis. KAOHSIUNG J MED SCI. 2023 JuRead more>>
- Zhiyuan Luo. et al. Quorum Sensing Interference Assisted Therapy-Based Magnetic Hyperthermia Amplifier for Synergistic Biofilm Treatment. SMALL. 2023 Sep;:2304836Read more>>
- Feng Qiu. et al. The mechanism of Chebulae Fructus Immaturus promote diabetic wound healing based on network pharmacology and experimental verification. J ETHNOPHARMACOL. 2024 Mar;322:117579Read more>>
- Hiroaki Eguchi. et al. Acrolein suppresses anticancer drug-induced toxicity mediated by activating claudin-1 and Nrf2 axis in a spheroid model of human lung squamous cell carcinoma cells. TOXICOL LETT. 2023 DecRead more>>
- Amei Tang. et al. Analyzing mechanisms of Qing Fei Bao Yuan Decoction granules in treating COPD based on LC-MS, network pharmacology and in vivo methods. Journal of Traditional and Complementary Medicine. 2024 ApRead more>>
- Yuan-yuan Zhang. et al. Modified Zhenwu Tang Delays Chronic Renal Failure Progression by Modulating Oxidative Stress and Hypoxic Responses in Renal Proximal Tubular Epithelial Cells. HELIYON. 2024 15Read more>>
- Sha Xu. et al. Approaches to selective and potent inhibition of glioblastoma by vanadyl complexes: Inducing mitotic catastrophe and methuosis. J INORG BIOCHEM. 2024 Aug;257:11261Read more>>
- Zetao Wang. et al. Nano-vibration exciter: Hypoxia-inducible factor 1 signaling pathway-mediated extracellular vesicles as bioactive glass substitutes for bone regeneration. BIOACT MATER. 2024 Oct;40:46Read more>>
- Xu Ke-Fei. et al. Hyperbaric oxygen enhances tumor penetration and accumulation of engineered bacteria for synergistic photothermal immunotherapy. NAT COMMUN. 2024 Jun;15(1):1-21Read more>>
- Jia-Jun Wu. et al. Heyingwuzi formulation alleviates diabetic retinopathy by promoting mitophagy via the HIF-1/BNIP3/NIX axis. WORLD J DIABETES. 2024 Jun 15; 15(6): 13171339Read more>>
- Yuko Mizukami. et al. Downregulation of chemoresistance by claudin-14 silencing in human colorectal cancer cells. ARCH BIOCHEM BIOPHYS. 2024 Aug;758:110075Read more>>
- Xiao-Hui Wang. et al. Schisandrin A can promote the anti-tumor effect of 5-Fu by reversing the immunosuppressive state of the body in rat. J ASIAN NAT PROD RES. 2024 09Read more>>
- Lizheng Wu. et al. Luoshi Neiyi Prescription inhibits estradiol synthesis and inflammation in endometriosis through the HIF1A/EZH2/SF-1 pathway. J ETHNOPHARMACOL. 2024 Dec;335:118659Read more>>
- Wenqi Chen. et al. Combined Exposure to High-Cholesterol Diet and PM2.5: Brain Injury and Regulatory Mechanism of HIF-1 in ApoE/ Female Mice. ATMOSPHERE-BASEL. 2024 Aug;15(8):952Read more>>
VALIDATION IMAGES
Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, Unconjugated (bs-20399R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei
Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, Unconjugated (bs-20399R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human A431 cell lysates; Lane 2: Human U-2OS cell lysates; Lane 3: Human HepG2 cell lysates probed with HIF-1 Alpha Polyclonal Antibody, Unconjugated (bs-20399R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human HepG2 cell lysates probed with HIF-1 Alpha Polyclonal Antibody, Unconjugated (bs-20399R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-20399R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-20399R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-20399R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-20399R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Recombinant human TNFSF13B protein, N-His(bs-43649P) probed with HIF-1 Alpha Polyclonal Antibody, Unconjugated (bs-20399R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.