bs-20398R [Primary Antibody]
HIF-1 Alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HIF-1 Alpha

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3091

Swiss Prot: Q16665

Source: KLH conjugated synthetic peptide derived from human HIF-1 Alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator(ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
Flow-Cyt(Flow-Cyt=1μg/Test)

Predicted Molecular Weight: 92 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cao et al. Danshensu, a major water-soluble component ofSalvia miltiorrhiza, enhances the radioresponse for Lewis Lung Carcinoma xenografts in mice. (2017) Oncol.Lett. 13:605-612Read more>>
  • Zhang P et al. MicroRNA-143a-3p modulates preadipocyte proliferation and differentiation by targeting MAPK7. Biomedicine & Pharmacotherapy, 108, 531–539. Read more>>
  • He Y et al. Association of Age with the Expression of Hypoxia-Inducible Factors HIF-1α, HIF-2α, HIF-3α and VEGF in Lung and Heart of Tibetan Sheep. Animals (Basel). 2019 Sep 11;9(9). pii: E673. Read more>>
  • Yan Liu. et al. Long noncoding RNA LINC00518 induces radioresistance by regulating glycolysis through an miR-33a-3p/HIF-1 negative feedback loop in melanoma. Cell Death Dis. 2021 Mar;12(3):1-19Read more>>
  • Chao Guo. et al. Long non-coding RNA muskelin 1 antisense RNA (MKLN1-AS) is a potential diagnostic and prognostic biomarker and therapeutic target for hepatocellular carcinoma. Exp Mol Pathol. 2021 Jun;120:104638Read more>>
  • Yanyu He. et al. Altered Hypoxia-Induced and Heat Shock Protein Immunostaining in Secondary Hair Follicles Associated with Changes in Altitude and Temperature in Tibetan Cashmere Goats. Animals-Basel. 2021 Oct;11(10):2798Read more>>
  • Longfei Xiao. et al. Dihydrotestosterone through blockade of TGF-/Smad signaling mediates the anti-fibrosis effect under hypoxia in canine Sertoli cells. J Steroid Biochem. 2022 Feb;216:106041Read more>>
  • Pan Cao. et al. Sirtuin1 attenuates acute liver failure by reducing reactive oxygen species via hypoxia inducible factor 1_. WORLD J GASTROENTERO. 2022 May 7; 28(17): 17981813Read more>>
  • Feng Junlong. et al. Explore the Effect of Asthma Regulating HIF-1 Pathway on Sperm Quality Based on Rat Model. BIOMED RES INT. 2022;2022:4194685Read more>>
  • Zihui Wang. et al. Heat stress and hypoxia inhibit the secretion of androgens and induce epithelial-to-mesenchymal transition associated with activated TGF-_/Smad signaling in canine cryptorchidism. REPROD DOMEST ANIM. 2022 Jun 09Read more>>
  • Huawei Wei. et al. Melatonin through blockade of Hif-1 signaling mediates the anti-fibrosis under hypoxia in canine Sertoli cells. REPROD BIOL. 2022 Dec;22:100677Read more>>
  • Ke Cheng. et al. IGF2BP3/HIF1A/YAP signaling plays a role in driving acute-on-chronic liver failure through activating hepatocyte reprogramming. CELL SIGNAL. 2023 Aug;108:110727Read more>>
VALIDATION IMAGES

Lane 1: Recombinant human HIF-1 Alpha protein, N-Trx-His(bs-42326P) probed with HIF-1 Alpha Polyclonal Antibody, Unconjugated (bs-20398R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.