Host: Rabbit
Target Protein: IL-10
Immunogen Range: 51-100/178
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3586
Swiss Prot: P22301
Source: KLH conjugated synthetic peptide derived from human IL-10
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
Predicted Molecular Weight: 20
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
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Paraformaldehyde-fixed, paraffin-embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Rat Blood Plasma; Lane 2: Rat Blood Plasma; Probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Raw264.7 Cells; Probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Recombinant human IL-10 protein, His (HEK293)(bs-43053P) probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.