bs-20355R-Cy5
[Conjugated Primary Antibody]
NFKB p65 Polyclonal Antibody, Cy5 Conjugated
DATASHEET
Host: Rabbit
Target Protein: NFKB p65
Immunogen Range: 201-300/551
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5970
Swiss Prot: Q04206
Source: KLH conjugated synthetic peptide derived from human NFKB p65
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
Conjugation: Cy5
Excitation/ Emission: 625,650nm/670nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(ICC)(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wu Z et al. Co-infection of Mycoplasma gallisepticum and Escherichia coli Triggers Inflammatory Injury Involving the IL-17 Signaling Pathway. Front Microbiol. 2019 Nov 15;10:2615.Read more>>
- Xiaoyan Hu. et al. Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death. Mol Med Rep. 2020 Sep;22(3):2003-2011Read more>>
- Yongli Zhang. et al. Licorice extract ameliorates hyperglycemia through reshaping gut microbiota structure and inhibiting TLR4/NF-_B signaling pathway in type 2 diabetic mice. Food Res Int. 2022 Mar;153:110945Read more>>
- Wenyang Wang. et al. Glycogen metabolism reprogramming promotes inflammation in coal dust-exposed lung. ECOTOX ENVIRON SAFE. 2022 Sep;242:113913Read more>>
- Jiaojiao Wu. et al. The involvement of Pellino-1 downregulation in the modulation of visceral hypersensitivity via the TLR4/NF-_B pathway in the rat fastigial nucleus. NEUROSCI LETT. 2022 Sep;787:136815Read more>>
- Guirong Chen. et al. The interaction of MD-2 with small molecules in huanglian jiedu decoction play a critical role in the treatment of sepsis.. FRONT PHARMACOL. 2022 Sep;13:947095-947095Read more>>
- Xuezhi Zhang. et al. Natural emodin reduces myocardial ischemia/reperfusion injury by modulating the RUNX1/miR?142?3p/DRD2 pathway and attenuating inflammation. EXP THER MED. 2022 Dec;24(6):1-11Read more>>
- Chen Zhang. et al. The novel hyaluronic acid granular hydrogel attenuates osteoarthritis progression by inhibiting the TLR-2/NF-B signaling pathway through suppressing cellular senescence. BIOENG TRANSL MED. 2022 Dec;:e10475Read more>>
- Yanjie Wang. et al. Inhibitory Effect of Bisdemethoxycurcumin on DNCB-Induced Atopic Dermatitis in Mice. MOLECULES. 2023 Jan;28(1):293Read more>>
- Gao Jie-Fang. et al. Myricetin treatment has ameliorative effects in DNFB-induced atopic dermatitis mice under high-fat conditions. TOXICOL SCI. 2022 DecRead more>>
- Yang Liu. et al. Isochlorogenic Acid C Restrains Erk/JNK/NF-B Signaling to Alleviate Inflammatory Response and Promote Cell Apoptosis. J FOOD BIOCHEM. 2023;2023:5547108Read more>>
- Fengjuan Chen. et al. JNK molecule is a toxic target for IPEC-J2 cell barrier damage induced by T-2 toxin. ECOTOX ENVIRON SAFE. 2023 Sep;263:115247Read more>>
- Jing Zhang. et al. Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-B and restoring lipid production in Caco-2 cell line. J FUNCT FOODS. 2023 Aug;107:105709Read more>>
- Xing Zeyu. et al. KPNA2 Silencing, Regulated by E3 Ubiquitin Ligase FBXW7, Alleviates Endothelial Dysfunction and Inflammation Through Inhibiting the Nuclear Translocation of p65 and IRF3: A Possible Therapeutic Approach for Atherosclerosis. INFLAMMATION. 2023 Jul;:1-18Read more>>
- Guoyong Zhang. et al. Buyang Huanwu Decoction suppresses cardiac inflammation and fibrosis in mice after myocardial infarction through inhibition of the TLR4 signalling pathway. J ETHNOPHARMACOL. 2023 Nov;:117388Read more>>
- Yuhan Lin. et al. Puerarin inhibits cisplatin-induced ototoxicity in mice through regulation of TRPV1-dependent calcium overload. BIOCHEM PHARMACOL. 2024 Feb;220:115962Read more>>
- Cong Zhang. et al. Effects of Se-enriched yeast on the amelioration of atrazine-induced meat quality degradation. FOOD CHEM. 2024 Oct;454:139737Read more>>
- Zhao Xu. et al. Sirt1 inhibits macrophage polarization and inflammation in gouty arthritis by inhibiting the MAPK/NF-B/AP-1 pathway and activating the Nrf2/HO-1 pathway. INFLAMM RES. 2024 May;:1-12Read more>>
- Jing Lian. et al. Acteoside and isoacteoside alleviate renal dysfunction and inflammation in lipopolysaccharide-induced acute kidney injuries through inhibition of NF-B signaling pathway. PLOS ONE. 2024 May;19(5):e030374Read more>>
- Mei Zhang. et al. Protective Effects of Resveratrol on Cytotoxicity of Mouse Hepatic Stellate Cells Induced by PM2.5. ATMOSPHERE-BASEL. 2024 May;15(5):588Read more>>
- Yudy Tjahjono. et al. 2-(3-(Chloromethyl)benzoyloxy)benzoic Acid Reduces Prostaglandin E-2 Concentration, NOX2 and NFKB Expression, ROS Production, and COX-2 Expression in Lipopolysaccharide-Induced Mice. PROSTAG OTH LIPID M. 2024 Jul;:106866Read more>>
- Qianli Tang. et al. Optical imaging detection of extracellular vesicles of miR-146 modified bone marrow mesenchymal stem cells promoting spinal cord injury repair. SLAS TECHNOL. 2024 Jul;:100172Read more>>
VALIDATION IMAGES