bs-2033R-BF594 [Conjugated Primary Antibody]
E.coli DH-5 Alpha Polyclonal Antibody, AbBy Fluor® 594 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: E.coli DH-5 Alpha

Clonality: Polyclonal

Isotype: IgG

Source: Whole cell protein of Ecoli DH-5 Alpha

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

This antibody reacts with Escherichia coli HD5a (E.coli HD5a), Escherichia coli is a gram-negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. E. coli DH5_ strain is an engineering strain widely used in biopharmaceuticals. According to the requirements of product quality inspection, it is necessary to detect the residual amount of DH5_ bacterial protein in biological products. This product is an anti-serum obtained by immunizing rabbits with DH5_ bacterial protein as an antigen, and purified by affinity chromatography to obtain antibody IgG, which can be used for ELISA, Western Blot and other experimental tests.

Conjugation: AbBy Fluor® 594

Excitation/ Emission: 590nm/617nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Bacteria
Others
(Escherichia coli)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Yiwei, et al. "Agarose-based microfluidic device for rapid point-of-care concentration and detection of pathogen." Analytical Chemistry (2014).Read more>>
  • entolhoda Heli. et al. Toward a nanopaper-based and solid phase immunoassay using FRET for the rapid detection of bacteria. Sci Rep-Uk. 2020 Sep;10(1):1-11Read more>>
  • Zachary D. Call. et al. Paper-based pump-free magnetophoresis. Anal Methods-Uk. 2020 Nov;12(43):5177-5185Read more>>
VALIDATION IMAGES