bs-20168R [Primary Antibody]
Phospho-Jak3 (Tyr785) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Phospho-Jak3 (Tyr785)

Modification Site: Tyr785

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3718

Swiss Prot: P52333

Source: KLH conjugated synthesised phosphopeptide derived from human Jak3 around the phosphorylation site of Tyr785

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 39


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yan Yao. et al. P38 modulates the lipid metabolism in non-alcoholic fatty liver disease by regulating the JAKCSTAT signaling pathway. FASEB J. 2022 Dec;37(1):e22716Read more>>
  • Liang Shao. et al. Betulonic acid regulates oviduct epithelial cell inflammation through the TLR4, MAPK, and JAK/STAT signalling pathways. REPROD FERT DEVELOP. 2023 May;35(8):480-491Read more>>
  • Ting Xiao. et al. Ameliorative effect of Alangium chinense (Lour.) Harms on rheumatoid arthritis by reducing autophagy with targeting regulate JAK3-STAT3 and COX-2 pathways. J ETHNOPHARMACOL. 2023 Sep;:117133Read more>>
VALIDATION IMAGES

Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Blood cell lysates probed with Phospho-Jak3 (Tyr785) Polyclonal Antibody, Unconjugated (bs-20168R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.