bs-20159R-PE [Conjugated Primary Antibody]
NFKB p65 Polyclonal Antibody, PE Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NFKB p65

Immunogen Range: 101-200/551


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5970

Swiss Prot: Q04206

Source: KLH conjugated synthetic peptide derived from human NFKB p65

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 61


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ding,et al.ZEB2 Attenuates LPS-Induced Inflammation by the NF-κB Pathway in HK-2 Cells.(2018) Inflammation. 41:722-731.Read more>>
  • Linyan Cheng. et al. Icariin attenuates thioacetamide_induced bone loss via the RANKL_p38/ERK_NFAT signaling pathway. Mol Med Rep. 2022 Apr;25(4):1-11Read more>>
  • Mohamed Abd El-Salam. et al. Novel antitumor activity of the combined treatment of galloylquinic acids from Copaifera lucens and doxorubicin in solid Ehrlich carcinoma-bearing mice via the modulation of the Notch signaling pathway. Life Sci. 2022 Mar;:120497Read more>>
  • Zhang Peng. et al. Study on the Mechanism of Bu-Shen-He-Mai Granules in Improving Renal Damage of Ageing Spontaneously Hypertensive Rats by Regulating Th17 Cell/Tregs Balance. EVID-BASED COMPL ALT. 2022;2022:8315503Read more>>
  • Ahmed H. Madkour. et al. Dose-dependent renoprotective impact of Lactoferrin against glycerol-induced rhabdomyolysis and acute kidney injury. LIFE SCI. 2022 May;:120646Read more>>
  • Aykut Ozturk. et al. The protective effects of trimetazidine against ovary ischemiareperfusion injury via the TLR4/Nf-kB signal pathway. J BIOCHEM MOL TOXIC. 2022 May 27Read more>>
  • Tian Li. et al. Maresin 1 improves cognitive decline and ameliorates inflammation and blood-brain barrier damage in rats with chronic cerebral hypoperfusion. BRAIN RES. 2022 Aug;1788:147936Read more>>
  • Franklin Ime Ani. et al. Infused aqueous curry tea extracts ameliorate N_-Nitro-L-Arginine Methyl Ester-induced liver dysfunction in male albino Wistar rats. J FOOD BIOCHEM. 2022 May 24Read more>>
  • El-Tanbouly, Ghada S.. et al. The emerging coloprotective effect of sildenafil against ulcerative colitis in rats via exerting counterbalance between NF-_B signaling and Nrf-2/HO-1 pathway. INFLAMMOPHARMACOLOGY. 2022 Aug;30(4):1351-1362Read more>>
  • Elshal, Mahmoud. et al. Escin suppresses immune cell infiltration and selectively modulates Nrf2/HO-1, TNF-/JNK, and IL-22/STAT3 signaling pathways in concanavalin A-induced autoimmune hepatitis in mice. INFLAMMOPHARMACOLOGY. 2022 Sep;:1-13Read more>>
  • Ming Dong Si. et al. Swertia mussotii prevents high-fat diet-induced non-alcoholic fatty liver disease in rats by inhibiting expression the TLR4/MyD88 and the phosphorylation of NF-B. PHARM BIOL. 2022 Oct 07Read more>>
  • Heba M. Hafez. et al. Agomelatine ameliorates cadmium-induced toxicity through the modification of HMGB-1/TLR-4/NFB pathway. TOXICOL APPL PHARM. 2022 Dec;457:116313Read more>>
  • Xiao Wang. et al. Kallistatin attenuates inflammatory response in rheumatoid arthritis via the NF-B signaling pathway. EUR J PHARMACOL. 2023 Jan;:17553Read more>>
  • Candan B.. et al. Beneficial Effects of Selenium on Kidney Injury via Nf-Kb and Aquaporin-1 Levels. BIOL TRACE ELEM RES. 2023 Nov;:1-9Read more>>
  • Li Ke. et al. The preventive effects of Lactobacillus casei 03 on Escherichia coli-induced mastitis in vitro and in vivo. J INFLAMM-LOND. 2024 Dec;21(1):1-9Read more>>
  • Adel Rana. et al. Oxidative stress and NF-KB/iNOS inflammatory pathway as innovative biomarkers for diagnosis of drowning and differentiating it from postmortem submersion in both fresh and saltwater in rats. INT J LEGAL MED. 2024 May;:1-16Read more>>
  • Elleithi Yomna. et al. Autophagy modulation attenuates sorafenib resistance in HCC induced in rats. CELL DEATH DIS. 2024 Aug;15(8):1-16Read more>>