bs-20157R [Primary Antibody]
HSV tag Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HSV tag

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide HSV tag

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The HSV (herpes simplex virus) epitope tag is frequently engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized HSV peptide epitope represents the amino acid sequence QPELAPEDPED. HSV Tag antibody can recognize C-terminal, internal, and N-terminal HSV-tagged proteins.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Cross Reactive Species: Tags
Others
(HSV tag)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

HSV-Tagged Fusion Protein Overexpression E.coli Lysates probed with HSV tag Polyclonall Antibody, Unconjugated (bs-20157R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.