bs-2013R [Primary Antibody]
Nrf2 (Ser40) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Nrf2 Ser40

Modification Site: Ser40

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4780

Swiss Prot: Q16236

Source: KLH conjugated synthetic phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40 [DF(p-S)QR]

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 68


Cross Reactive Species: Human
Mouse
Rat
Pig
Others

Predicted Cross Reactive Species: Dog
Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mo, Chunfen, et al. "The Crosstalk between Nrf2 and AMPK Pathways Is Important for the Anti-inflammatory Effect of Berberine in LPS-Stimulated Macrophages and Endotoxin-Shocked Mice."?Antioxidants & redox signaling?ja (2013).Read more>>
  • Lee, Bao-Hong, et al. "Dimerumic acid attenuates receptor for advanced glycation endproduct (RAGE) signal to inhibit inflammation and diabetes mediated by Nrf2 activation and promoted methylglyoxal metabolism into D-lactate acid" Free Radical Biology and MedicineRead more>>
  • Lee, Bao-Hong, et al. "Ankaflavin: A natural novel PPAR?? agonist up-regulated Nrf2 to attenuate methylglyoxal (MG)-induced diabetes< i> in vivo</i>."Free Radical Biology and Medicine (2012).yRead more>>
  • Patwardhan, R. S., et al. "Mitigation of radiation induced hematopoietic injury via regulation of cellular MAPK/phosphatase levels and increasing hematopoietic stem cells." Free Radical Biology and Medicine (2013).Read more>>
  • Bao-Hong, L. et al. "Graptopetalum paraguayense and resveratrol ameliorates 3 carboxymethyllysine (CML)-induced pancreas dysfunction and 4 hyperglycemia." Food and Chemical Toxicology. (2013).Read more>>
  • Lee, Bao-Hong, et al. "< i> Graptopetalum paraguayense</i> and resveratrol ameliorates carboxymethyllysine (CML)-induced pancreas dysfunction and hyperglycemia." Food and Chemical Toxicology 62 (2013): 492-498.Read more>>
  • Hsu, Wei-Hsuan, et al. "Monascin and AITC attenuate methylglyoxal-induced PPAR?? phosphorylation and degradation through inhibition of the oxidative stress/PKC pathway depending on Nrf2 activation."Journal of agricultural and food chemistry (2013)..Read more>>
  • Cheng, An-Sheng, Yu-Hsiang Cheng, and Tsu-Liang Chang. "Resveratrol protects RINm5F pancreatic cells from methylglyoxal-induced apoptosis." Journal of Functional Foods (2013).Read more>>
  • Kakehashi A, Kato A, Ishii N, Wei M, Morimura K, et al. (2014) Valerian Inhibits Rat Hepatocarcinogenesis by Activating GABA(A) Receptor-Mediated Signaling. PLoS ONE 9(11): e113610.Read more>>
  • Kaneko, Yoko S., et al. "Aripiprazole increases NAD (P) H–quinone oxidoreductase-1 and heme oxygenase-1 in PC12 cells." Journal of Neural Transmission: 1-16.Read more>>
  • Chang, Wen-Chang, et al. "Scopoletin Protects against Methylglyoxal-Induced Hyperglycemia and Insulin Resistance Mediated by Suppression of Advanced Glycation Endproducts (AGEs) Generation and Anti-Glycation." Molecules 20.2 (2015): 2786-2801.Read more>>
  • Cárdeno, Ana, et al. "Squalene targets pro-and anti-inflammatory mediators and pathways to modulate over-activation of neutrophils, monocytes and macrophages." Journal of Functional Foods 14 (2015): 779-790.Read more>>
  • Santofimia-Castaño, Patricia, et al. "Melatonin induces the expression of Nrf2-regulated antioxidant enzymes via PKC and Ca 2+ influx activation in mouse pancreatic acinar cells." Free Radical Biology and Medicine (2015).Read more>>
  • Allard, Joanne S., et al. "Prolonged metformin treatment leads to reduced transcription of Nrf2 and neurotrophic factors without cognitive impairment in older C57BL/6J mice." Behavioural Brain Research (2016).Read more>>
  • Yen, Ting-Lin, et al. "Andrographolide stimulates p38 MAPK-Nrf2-HO-1 signaling in primary cerebral endothelial cells for definite protection against ischemic stroke in rats." Translational Research (2016).Read more>>
  • Ko, Su Hyuk, et al. "Crude Preparations of Helicobacter pylori Outer Membrane Vesicles Induce Upregulation of Heme Oxygenase-1 via Activating Akt-Nrf2 and mTOR-IκB Kinase-NF-κB Pathways in Dendritic Cells." Infection and Immunity (2016): IAI-00190.Read more>>
  • Ko, Su Hyuk, et al. "Bacteroides fragilis Enterotoxin Upregulates Heme Oxygenase-1 in Intestinal Epithelial Cells via a Mitogen-Activated Protein Kinases-and NF-κB-Dependent Pathway, Leading to Modulation of Apoptosis." Infection and Immunity (2016): IAI-00191.Read more>>
  • Kim, Mi-Hwi, et al. "EX4 stabilizes and activates Nrf2 via PKCδ, contributing to the prevention of oxidative stress-induced pancreatic beta cell damage." Toxicology and Applied Pharmacology (2016).Read more>>
  • Hou, Xiang, et al. "Ameliorative effect of ampelopsin on LPS-induced acute phase response in piglets." Journal of Functional Foods 35 (2017): 489-498.Read more>>
  • Ghosh, Anindya, et al. "Insulin Inhibits Nrf2 Gene Expression via Heterogeneous Nuclear Ribonucleoprotein F/K in Diabetic Mice." Endocrinology 158.4 (2017): 903-919.Read more>>
  • Cheng et al. Resveratrol protects against methylglyoxal-induced hyperglycemia and pancreatic damage in vivo. (2015) Nutrient. 7:2850-65Read more>>
  • Zamponi, E., et al. “Nrf2 stabilization prevents critical oxidative damage in Down syndrome cells.” Aging Cell (2018); e12812.Read more>>
  • Bhatt, NP., et al. “Apocynin protects mesangial cells from lipopolysaccharide-induced inflammation by exerting heme oxygenase 1-mediated monocyte chemoattractant protein-1 suppression.” International Journal of Molecular Medicine (2017); 40(4): 1294-1301.Read more>>
  • Shen, Joseph YuHung. “PATHOPHYSIOLOGY OF THE CYCLICAL EPIDERMOLYTIC PALMOPLANTAR KERATODERMA (EPPK) IN THE KERATIN 9 MOUSE MODEL.” John Hopkins University (2017); Graduate Thesis.Read more>>
  • Zamponi et al. Nrf2 stabilization prevents critical oxidative damage in Down syndrome cells. (2018) Aging.Cell. 17:e12812Read more>>
  • Gęgotek A et al. Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation. Arch Dermatol Res. 2019 Apr;311(3):203-219. Read more>>
  • Ko SH et al. Bacteroides fragilis enterotoxin upregulates heme oxygenase-1 in dendritic cells via reactive oxygen species-, mitogen-activated protein kinase-, and Nrf2-dependent pathway. World J Gastroenterol. 2020 Jan 21;26(3):291-306.Read more>>
  • Gong H et al. Nrf2-SHP Cascade-Mediated STAT3 Inactivation Contributes to AMPK-Driven Protection Against Endotoxic Inflammation. Front Immunol. 2020 Mar 10;11:414.Read more>>
  • Ko SH et al. Bacteroides fragilis enterotoxin upregulates heme oxygenase-1 in dendritic cells via reactive oxygen species-, mitogen-activated protein kinase-, and Nrf2-dependent pathway. World J Gastroenterol. 2020 Jan 21;26(3):291-306.Read more>>
  • Jong Ik Jeonet al. Bacteroides fragilis Enterotoxin Induces Sulfiredoxin-1 Expression in Intestinal Epithelial Cell Lines Through a Mitogen-Activated Protein Kinases- and Nrf2-Dependent Pathway, Leading to the Suppression of Apoptosis. Int J Mol Sci. 2020 Jul 29;21(15):5383.Read more>>
  • Marina K et al. Mesenchymal Stem Cells Early Response to Low-Dose Ionizing RadiationFront Cell Dev Biol.2020 Dec 14;8:584497.Read more>>
  • Szilamr Ferenczi. et al. Effects of Single and Repeated Oral Doses of Ochratoxin A on the Lipid Peroxidation and Antioxidant Defense Systems in Mouse Kidneys. Toxins. 2020 Nov;12(11):732Read more>>
  • Ibtissem Rahim. et al. Melatonin alleviates sepsis-induced heart injury through activating the Nrf2 pathway and inhibiting the NLRP3 inflammasome. N-S Arch Pharmacol. 2021 Feb;394(2):261-277Read more>>
  • Wiebke Sachs. et al. Distinct Modes of Balancing Glomerular Cell Proteostasis in Mucolipidosis Type II and III Prevent Proteinuria. J Am Soc Nephrol. 2020 Aug;31(8):1796-1814Read more>>
  • Michelle L. Kerns. et al. Pathogenic and therapeutic role for NRF2 signaling in ultraviolet lightCinduced skin pigmentation. Jci Insight. 2020 Oct 15; 5(20): e139342Read more>>
  • Jianbing Wu. et al. Design, Synthesis, and Biological Evaluation of Organic Nitrite (NO2C) Donors as Potential Anticerebral Ischemia Agents. J Med Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Ziling Wang. et al. Ginsenoside Rg1 prevents bone marrow mesenchymal stem cell senescence via NRF2 and PI3K/Akt signaling. Free Radical Bio Med. 2021 AugRead more>>
  • Hao Huang. et al. Maintaining blood retinal barrier homeostasis to attenuate retinal ischemia-reperfusion injury by targeting the KEAP1/NRF2/ARE pathway with lycopene. Cell Signal. 2021 Dec;88:110153Read more>>
  • Ana L. Vivinetto. et al. Myelin-associated glycoprotein activation triggers glutamate uptake by oligodendrocytes in vitro and contributes to ameliorate glutamate-mediated toxicity in vivo. Bba-Mol Basis Dis. 2022 Apr;1868:166324Read more>>
  • Ming Qi. et al. Ellagic acid ameliorates paraquat-induced liver injury associated with improved gut microbial profile. Environ Pollut. 2022 Jan;293:118572Read more>>
  • Cheng-You Lu. et al. Calycosin alleviates H2O2-induced astrocyte injury by restricting oxidative stress through the Akt/Nrf2/HO-1 signaling pathway. 2022 Jan 06Read more>>
  • Yunxiao Zhong. et al. Nrf2 Inhibits the Progression of Parkinson&rsquo;s Disease by Upregulating AABR07032261.5 to Repress Pyroptosis. J Inflamm Res. 2022 Feb;15:669-685Read more>>
  • Ning Zhou. et al. Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2. MOLECULES. 2022 Jan;27(11):3542Read more>>
  • Kim, Da-Eun. et al. Plk2-mediated phosphorylation and translocalization of Nrf2 activates anti-inflammation through p53/Plk2/p21cip1 signaling in acute kidney injury. CELL BIOL TOXICOL. 2022 Jul;:1-21Read more>>
  • Jhong-Kuei Chen. et al. Artemisia argyi extract ameliorates IL-17A-induced inflammatory response by regulation of NF-B and Nrf2 expression in HIG-82 synoviocytes. ENVIRON TOXICOL. 2022 AugRead more>>
  • Jiying Hou. et al. Ginsenoside Rg1 Reduces Oxidative Stress Via Nrf2 Activation to Regulate Age-Related Mesenchymal Stem Cells Fate Switch Between Osteoblasts and Adipocytes. EVID-BASED COMPL ALT. 2022 Oct 11;2022:1411354Read more>>
  • Xueling Zhang. et al. Type 2 Diabetes Mellitus Makes Corneal Endothelial Cells Vulnerable to Ultraviolet A-Induced Oxidative Damage Via Decreased DJ-1/Nrf2/NQO1 Pathway. INVEST OPHTH VIS SCI. 2022 Nov;63(12):25-25Read more>>
  • Martin Molinero, Glenda Daniela. et al. A soybean based-diet prevents Cadmium access to rat cerebellum, maintaining trace elements homeostasis and avoiding morphological alterations. BIOMETALS. 2022 Nov;:1-3Read more>>
  • Ye Yang. et al. Neocryptotanshinone protects against myocardial ischemia-reperfusion injury by promoting autolysosome degradation of protein aggregates via the ERK1/2-Nrf2-LAMP2 pathway. PHYTOMEDICINE. 2023 Feb;110:154625Read more>>
  • Rui Ji. et al. Carnosol inhibits KGN cells oxidative stress and apoptosis and attenuates polycystic ovary syndrome phenotypes in mice through Keap1-mediated Nrf2/HO-1 activation. PHYTOTHER RES. 2023 FebRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (bs-2013R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody and DAPIstaining


Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (bs-2013R), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody


L1 mouse heart, L2 mouse muscle lysates probed (bs-2013R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. The specific protein band has also been reported to migrate to around 100kDa.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (bs-2013R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (bs-2013R) at 1:400 overnight at 4°C, DAB staining.


HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-Nrf2 (Ser40)) polyclonal Antibody, Unconjugated (bs-2013R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Lane 1: Human Hela cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Jurkat cell lysates ; Lane 5: Human B16 cell lysates ; Lane 6: Human A549 cell lysates probed with Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (bs-2013R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.