Host: Rabbit
Target Protein: EGF
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1950
Swiss Prot: P01133
Source: KLH conjugated synthetic peptide derived from human EGF
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:50-200)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 5.8
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
- Bi, Lingyun, et al. "Effect of bone marrow stem cell mobilisation on the expression levels of cellular growth factors in a rat model of acute tubular necrosis." Experimental and Therapeutic Medicine.Read more>>
- Dapeng Yang. et al. The histologic comparison of submandibular gland between yak and yellow cattle. ANAT HISTOL EMBRYOL. 2023 ApRead more>>
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with EGF Polyclonal Antibody(bs-2008R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (EGF) polyclonal Antibody, Unconjugated (bs-2008R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.