bs-2006R [Primary Antibody]
PCNA-Proliferation Marker Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PCNA-proliferation Marker

Immunogen Range: 151-261/261


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5111

Swiss Prot: P12004

Source: KLH conjugated synthetic peptide derived from human PCNA

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 29


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Pig
Chicken
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liang, Sixian, et al. "Silencing of CXCR4 sensitizes triple-negative breast cancer cells to cisplatin." Oncotarget 6.2 (2015): 1020-1030.Read more>>
  • Ji, Wei, et al. "Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells." Molecular Medicine Reports.Read more>>
  • Zhao et al. Adipose differentiation‑related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation. (2017) Mol.Med.Re. 16:3079-3086Read more>>
  • Zhao et al. Adipose differentiation‑related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation. (2017) Mol.Med.Re. 16:3079-3086Read more>>
  • Zhou et al. Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway. (2018) Int.J.Mol.Med. 41:473-484Read more>>
  • Chai et al. Hypoxia induces pulmonary arterial fibroblast proliferation, migration, differentiation and vascular remodeling via the PI3K/Akt/p70S6K signaling pathway. (2018) Int.J.Mol.Med. 41:2461-2472Read more>>
  • Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203.Read more>>
  • Jolly,et al.Targeted endothelial gene deletion of Triggering Receptor Expressed on Myeloid cells-1 protects mice during septic shock.(2018) Cardiovascular Research. 114:907-918.Read more>>
  • Zhang,et al.Lysosomal deposition of copper oxide nanoparticles triggers HUVEC cells death.(2018) Biomaterials. 161:228-239.Read more>>
  • Wang C et al. 4-Amino-2-trifluoromethyl-phenyl retinate induced differentiation of human myelodysplastic syndromes SKM-1 cell lines by up-regulating DDX23. Biomed Pharmacother. 2019 Dec 16;123:109736.Read more>>
  • Jin J et al. A novel S1P1 modulator IMMH002 ameliorates psoriasis in multiple animal models. Acta Pharmaceutica Sinica B. 2019.Read more>>
  • Yang Zhang. et al. Plumbagin Inhibits Proliferation, Migration, and Invasion of Retinal Pigment Epithelial Cells Induced by FGF-2. Tissue Cell. 2021 Oct;72:101547Read more>>
  • Qing Li. et al. Fumonisin B1 Inhibits Cell Proliferation and Decreases Barrier Function of Swine Umbilical Vein Endothelial Cells. Toxins. 2021 Dec;13(12):863Read more>>
  • Yu, Ting-Ting. et al. Harnessing chlorin e6 loaded by functionalized iron oxide nanoparticles linked with glucose for target photodynamic therapy and improving of the immunogenicity of lung cancer. J Cancer Res Clin. 2022 Jan;:1-13Read more>>
  • Ning Han. et al. Ferroptosis triggered by dihydroartemisinin facilitates chlorin e6 induced photodynamic therapy by inhibiting GPX4 and enhancing ROS. Eur J Pharmacol. 2022 Feb;:174797Read more>>
  • Wan Boyang. et al. Zearalenone promotes follicle development through activating SIRT1/PGC-1_ signaling pathway in the ovaries of weaned gilts. J Anim Sci. 2022 FebRead more>>
  • Suihui Li. et al. Hsa_circ_0048674 facilitates hepatocellular carcinoma progression and natural killer cell exhaustion depending on the regulation of miR-223-3p/PDL1. Histol Histopathol. 2022 Feb 21;1844Read more>>
  • Yu TT. et al. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.. Front Pharmacol. 2022 Mar;13:837784-837784Read more>>
  • Yang, Xiao-Xin. et al. A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin. J NANOBIOTECHNOL. 2022 Dec;20(1):1-19Read more>>
  • Ma, Wenbin. et al. CircPCNX Promotes PDGF-BB-Induced Proliferation and Migration of Human Aortic Vascular Smooth Muscle Cells Through Regulating miR-1278/DNMT1 Axis. CARDIOVASC DRUG THER. 2022 Jun;:1-13Read more>>
  • Cong Lan. et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBIOMEDICINE. 2022 Aug;82:104139Read more>>
  • Jiufang Cao. et al. Rapamycin inhibits the progression of human acute myeloid leukemia by regulating circ_0094100/miR-217/ATP1B1 axis. EXP HEMATOL. 2022 JulRead more>>
  • Guangdong Bai. et al. Perinatal exposure to glyphosate-based herbicides impairs progeny health and placental angiogenesis by disturbing mitochondrial function. ENVIRON INT. 2022 Dec;170:107579Read more>>
  • Jijiong Zhang. et al. CircRNA hsa_circ_0075048 promotes the malignant progression of non-small cell lung cancer by up-regulating HMGB2 expression via targeting miR-1225-5p.. HISTOL HISTOPATHOL. 2022 Nov;:18551-18551Read more>>
  • Guangdong Bai. et al. Perinatal exposure to high concentration glyphosate-based herbicides induces intestinal apoptosis by activating endoplasmic reticulum stress in offspring. SCI TOTAL ENVIRON. 2023 Mar;865:161223Read more>>
  • Yu Xiong. et al. Luteolin Isolated from <i>Polygonum cuspidatum</i> Is a Potential Compound against Nasopharyngeal Carcinoma. BIOMED RES INT. 2022 Dec 23;2022:9740066Read more>>
  • Xiaoqing Song. et al. Protective effect of hydroxysafflor yellow A on MSCs against senescence induced by D-galactose. CHIN HERB MED. 2022 DecRead more>>
  • Ning Han. et al. Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy. PHYTOMEDICINE. 2023 Jan;:154682Read more>>
  • Zhongqing Liu. et al. Crocetin Regulates Functions of Neural Stem Cells to Generate New Neurons for Cerebral Ischemia Recovery. ADV HEALTHC MATER. 2023 Mar;:2203132Read more>>
  • Siqi Liu. et al. Estrogen-mediated oar-miR-485-5p targets PPP1R13B to regulate myoblast proliferation in sheep. INT J BIOL MACROMOL. 2023 May;236:123987Read more>>
  • Zheng Kou. et al. Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing. INT J MOL SCI. 2023 Jan;24(10):8688Read more>>
  • Zhang Shuai. et al. Aqueous M. oleifera leaf extract alleviates DSS-induced colitis in mice through suppression of inflammation. J ETHNOPHARMACOL. 2023 Jul;:116929Read more>>
  • Jie Su. et al. Study of spermatogenic and Sertoli cells in the Hu sheep testes at different developmental stages. FASEB J. 2023 Jul;37(8):e23084Read more>>
  • Chun Huang. et al. Whole-transcriptome analysis of longissimus dorsi muscle in cattle-yaks reveals the regulatory functions of ADAMTS6 gene in myoblasts. INT J BIOL MACROMOL. 2024 Mar;262:129985Read more>>
  • Hui Yuan-Jian. et al. B-Myb deficiency boosts bortezomib-induced immunogenic cell death in colorectal cancer. SCI REP-UK. 2024 Apr;14(1):1-15Read more>>
  • Li Liu-Gen. et al. Dihydroartemisinin-driven selective anti-lung cancer proliferation by binding to EGFR and inhibition of NRAS signaling pathway-induced DNA damage. SCI REP-UK. 2024 May;14(1):1-15Read more>>
  • Wen Yu Zhang. et al. AMPK regulates immature boar Sertoli cell proliferation through affecting CDK4/Cyclin D3 pathway and mitochondrial function. THERIOGENOLOGY. 2024 Aug;224:9Read more>>
  • Hong Yinwen. et al. Loureirin B inhibits Cervical Cancer Development by Blocking PI3K/AKT Signaling Pathway: Network Pharmacology Analysis and Experimental Validation. APPL BIOCHEM BIOTECH. 2024 Jun;:1-18Read more>>
  • Xiao-Feng Bai. et al. Cepharanthine triggers ferroptosis through inhibition of NRF2 for robust ER stress against lung cancer. EUR J PHARMACOL. 2024 Sep;979:176839Read more>>
  • Junfeng He. et al. Effect of the TGF-/BMP Signaling Pathway on the Proliferation of Yak Pulmonary Artery Smooth Muscle Cells under Hypoxic Conditions. ANIMALS. 2024 Jan;14(14):2072Read more>>
  • Chen Hsien-Chung. et al. Progesterone boosts abiraterone-driven target and NK cell therapies against glioblastoma. J EXP CLIN CANC RES. 2024 Dec;43(1):1-17Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti- PCNA-Proliferation Marker Polyclonal Antibody, Unconjugated (bs-2006R) at 1:100 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human brain tissue labeled with Anti-PCNA Polyclonal Antibody, Unconjugated (bs-2006R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-PCNA Polyclonal Antibody, Unconjugated (bs-2006R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse spleen lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-2006R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PCNA-Proliferation Marker Polyclonal Antibody, Unconjugated (bs-2006R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Human Hela cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Human HepG2 cell lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-2006R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA) Polyclonal Antibody, Unconjugated (bs-2006R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA) Polyclonal Antibody, Unconjugated (bs-2006R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA) Polyclonal Antibody, Unconjugated (bs-2006R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA) Polyclonal Antibody, Unconjugated (bs-2006R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA) Polyclonal Antibody, Unconjugated (bs-2006R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA) Polyclonal Antibody, Unconjugated (bs-2006R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.