bs-1990R [Primary Antibody]
MITF Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MITF

Immunogen Range: 351-450/526


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4286

Swiss Prot: O75030

Source: KLH conjugated synthetic peptide derived from human MITF

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 59


Cross Reactive Species: Mouse
Others

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Han, Joon‐Seung, Jong Hwan Sung, and Seung Kwon Lee. "Antimelanogenesis Activity of Hydrolyzed Ginseng Extract (GINST) via Inhibition of JNK Mitogen‐activated Protein Kinase in B16F10 Cells." Journal of Food Science (2016).Read more>>
  • Kito, Yusuke, Chiemi Saigo, and Tamotsu Takeuchi. "Novel Transgenic Mouse Model of Polycystic Kidney Disease." The American Journal of Pathology (2017).Read more>>
  • Rice et al. Melanoblast development coincides with the late emerging cells from the dorsal neural tube in turtle Trachemys scripta. (2017) Sci.Rep. 7:12063Read more>>
  • Egawa et al. Therapeutic potential of CPI-613 for targeting tumorous mitochondrial energy metabolism and inhibiting autophagy in clear cell sarcoma. (2018) PLoS.One. 13:e0198940Read more>>
  • Qinyu Ma. et al. Small extracellular vesicles deliver osteolytic effectors and mediate cancer\induced osteolysis in bone metastatic niche. J Extracell Vesicles. 2021 Feb;10(4):e12068Read more>>
  • Ting Yang. et al. Combine with RNA-seq Reveals the Effect of Melatonin in the Synthesis of Melanin in Primary Melanocytes of Silky Fowls Black-Bone Chicken. GENES-BASEL. 2023 Aug;14(8):1648Read more>>
  • Ruiting Li. et al. Effects of SLC45A2 and GPNMB on Melanin Deposition Based on Transcriptome Sequencing in Chicken Feather Follicles. ANIMALS. 2023 Jan;13(16):2608Read more>>
VALIDATION IMAGES

Lane 1: mouse brain carcinoma lysates Lane 2: mouse kidney lysates probed with Anti MITF Polyclonal Antibody, Unconjugated (bs-1990R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 58kD. Observed band size: 58kD.


Formalin-fixed and paraffin embedded mouse melanoma labeled with Anti- MITF Polyclonal Antibody, Unconjugated (bs-1990R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse melanoma labeled with Anti- MITF Polyclonal Antibody, Unconjugated (bs-1990R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C and DAPI\n