bs-1970R [Primary Antibody]
LAMP-1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LAMP-1

Immunogen Range: 301-417/417


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3916

Swiss Prot: P11279

Source: KLH conjugated synthetic peptide derived from human LAMP-1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 42


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jeong, Keun-Yeong, et al. "Combination of macrophage inflammatory protein 1 alpha with existing therapies to enhance the antitumor effects on murine hepatoma." Journal of Radiation Research (2014): rru077.Read more>>
  • Guo, Hong, et al. "Niemann Pick type C2 deficiency impairs autophagy-lysosomal activity, mitochondrial function, and TLR signaling in adipocytes."Journal of Lipid Research (2016): jlr-M066522.Read more>>
  • Sun, Ming-Xia, et al. "Porcine reproductive and respiratory syndrome virus induces autophagy to promote virus replication." Autophagy 8.10 (2012): 1434-1447.Read more>>
  • Bechor, Sapir, et al. "Adipose tissue conditioned media support macrophage lipid-droplet biogenesis by interfering with autophagic flux." Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2017).Read more>>
  • Pan, Pan, et al. "Black Raspberries Enhance Natural Killer Cell Infiltration into the Colon and Suppress the Progression of Colorectal Cancer." Frontiers in Immunology 8 (2017): 997.Read more>>
  • Dixit, Saurabh, et al. "Caveolin-mediated endocytosis of the Chlamydia M278 outer membrane peptide encapsulated in poly (lactic acid)-Poly (ethylene glycol) nanoparticles by mouse primary dendritic cells enhances specific immune effectors mediated by MHC class II and CD4+ T cells." Biomaterials (2017).Read more>>
  • Pan et al. Black Raspberries Enhance Natural Killer Cell Infiltration into the Colon and Suppress the Progression of Colorectal Cancer. (2017) Front.Immunol. 8:997Read more>>
  • Goreczny et al. Hic-5 regulates fibrillar adhesion formation to control tumor extracellular matrix remodeling through interaction with tensin1. (2018) Oncogene. 37:1699-1713Read more>>
  • Hirohito Hirata. et al. PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking. Faseb J. 2021 Feb;35(2):e21281Read more>>
  • Xiaoshuang Zuo. et al. Photobiomodulation and diffusing optical fiber on spinal cords impact on nerve cells from normal spinal cord tissue in piglets. 2021 Jan 02Read more>>
  • Mingxing Fang. et al. SOX11 and FAK participate in the stretch?induced mechanical injury to alveolar type?2 epithelial cells. Int J Mol Med. 2021 Jan;47(1):361-373Read more>>
  • Ji Eun Won. et al. Immune checkpoint silencing using RNAi-incorporated nanoparticles enhances antitumor immunity and therapeutic efficacy compared with antibody-based approaches. J Immunother Cancer. 2022 Feb;10(2):e003928Read more>>
  • Shiro Jimi. et al. Intracellular Survival of Biofilm-Forming MRSA OJ-1 by Escaping from the Lysosome and Autophagosome in J774A Cells Cultured in Overdosed Vancomycin. Microorganisms. 2022 Feb;10(2):348Read more>>
  • Peng, Jialing. et al. Morphine-induced microglial immunosuppression via activation of insufficient mitophagy regulated by NLRX1. J NEUROINFLAMM. 2022 Dec;19(1):1-21Read more>>
  • Colin T. Shearn. et al. The autophagic protein p62 is a target of reactive aldehydes in human and murine cholestatic liver disease. PLOS ONE. 2022 Nov;17(11):e0276879Read more>>
  • Shu-wen Xu. et al. Cigarette smoke extract-induced inflammatory response via inhibition of the TFEB-mediated autophagy in NR8383 cells. EXP LUNG RES. 2023 Jan 13Read more>>
  • Lili Fan. et al. Homoplantaginin attenuates high glucose-induced vascular endothelial cell apoptosis through promoting autophagy via the AMPK/TFEB pathway. PHYTOTHER RES. 2023 MaRead more>>
  • Yuko Naya. et al. Pathological study of proximal tubule mitochondria in diclofenac-induced acute kidney injury model mice. TISSUE CELL. 2023 Jul;:102188Read more>>
VALIDATION IMAGES

Image provided by One World Lab validation initiative. PC3 cells were stained with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at [1:100] in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT.


Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.


RSC96 probed with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Human U937 cells probed with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).


Formalin-fixed and paraffin embedded rat kidney labeled with Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


RSC96 cells probed with LAMP-1 Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-1970R-A647) at 1:100 for 30 minutes compared to control cells (blue)and isotype control (orange).


Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:400 overnight at 4°C, DAB staining.


A549 cells were stained with LAMP-1 Polyclonal Antibody, Unconjugated(bs-1970R) at 1:100 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


Lane 1: Human HT-1080 cell lysates; Lane 2: Human JAR cell lysates probed with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LAMP-1) Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LAMP-1) Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LAMP-1) Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LAMP-1) Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Human HT-1080 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human JAR cell lysates probed with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.


4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (LAMP1) polyclonal Antibody, unconjugated (bs-1970R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.


Jurkat cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with LAMP1 Antibody(bs-1970R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.