bs-1948R-PerCP [Conjugated Primary Antibody]
WNT5A Polyclonal Antibody, PerCP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: WNT5A

Immunogen Range: 301-381/381


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7474

Swiss Prot: P41221

Source: KLH conjugated synthetic peptide derived from human WNT5A

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.

Conjugation: PerCP

Excitation/ Emission: 482nm/677nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 35


Cross Reactive Species: Human
Mouse
Rat
Dog

Predicted Cross Reactive Species: Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Król, Magdalena, et al. "Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors." PloS one 9.1 (2014): e83995.Read more>>
  • Saha, Subhrajit, et al. "Macrophage-derived extracellular vesicle-packaged WNTs rescue intestinal stem cells and enhance survival after radiation injury." Nature Communications 7 (2016).Read more>>
  • Guo, Furong, et al. "Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease." Experimental and Therapeutic Medicine.(2017)Read more>>
  • Guo et al. Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease. (2017) Exp.Ther.Me. 14:4231-4237Read more>>
  • Zhao L et al. Reducing macrophage numbers alleviates temporomandibular joint ankylosis. Cell Tissue Res. 2019 Sep 14.Read more>>
  • Hai Zhao. et al. Oxidative stress caused by a dysregulated Wnt/-catenin signalling pathway is involved in abnormal placenta formation in pregnant mice with chronic fatigue syndrome. Zygote. 2020 Oct;:1-8Read more>>
  • Ji, Xiang-Fen. et al. Noncanonical Wnt5a/JNK Signaling Contributes to the Development of D-Gal/LPS-Induced Acute Liver Failure. Inflammation. 2022 Jan;:1-12Read more>>
  • Jianyu Li. et al. MicroRNA-200a regulates skin pigmentation by targeting WNT5A and FZD4 in Cashmere goats. RES VET SCI. Res Vet Sci. 2022 ApRead more>>
  • Huan He. et al. BDE-209 disturbed proliferation and differentiation of spermatogonia during mitotic process through estrogen receptor . REPROD BIOL. 2023 Jun;23:100737Read more>>
  • Yuanchao Zhu. et al. Biomimetic Porous Magnesium Alloy Scaffolds Promote the Repair of Osteoporotic Bone Defects in Rats through Activating the Wnt/-Catenin Signaling Pathway. ACS BIOMATER-SCI ENG. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Yugui Du. et al. Role of mechanosensitive ion channel Piezo1 in tension-side orthodontic alveolar bone remodeling in rats. ARCH ORAL BIOL. 2023 Nov;155:105798Read more>>
  • Na Sun. et al. Scutellarin targets Wnt5a against zearalenone-induced apoptosis in mouse granulosa cells in vitro and in vivo. J HAZARD MATER. 2023 Nov;:132917Read more>>
  • Ji Xiang-Fen. et al. Associations of Wnt5a expression with liver injury in chronic hepatitis B virus infection. BMC INFECT DIS. 2023 Dec;23(1):1-11Read more>>
  • Ippei Horibe. et al. Acquired curved hair is caused by fusion of multiple hair matrix cells. J DERMATOL SCI. 2024 FebRead more>>
VALIDATION IMAGES