bs-19359R [Primary Antibody]
SAA1/Serum Amyloid A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SAA1/Serum Amyloid A

Immunogen Range: 41-94/122


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6288

Swiss Prot: P0DJI8

Source: KLH conjugated synthetic peptide derived from human SAA1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Jun 2012]

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)

Predicted Molecular Weight: 14 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kangxi Cao. et al. SAA1 Expression as a Potential Prognostic Marker of the Tumor Microenvironment in Glioblastoma. FRONT NEUROL. 2022; 13: 905561Read more>>
  • Xin Shiyong. et al. Identification of a risk model for prognostic and therapeutic prediction in renal cell carcinoma based on infiltrating M0 cells. SCI REP-UK. 2024 Jun;14(1):1-19Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SAA1/Serum Amyloid A Polyclonal Antibody, Unconjugated (bs-19359R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SAA1/Serum Amyloid A) Polyclonal Antibody, Unconjugated (bs-19359R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SAA1/Serum Amyloid A) Polyclonal Antibody, Unconjugated (bs-19359R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining


Paraformaldehyde-fixed, paraffin embedded (human prostate carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SAA1/Serum Amyloid A) Polyclonal Antibody, Unconjugated (bs-19359R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining


Paraformaldehyde-fixed, paraffin embedded (human small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SAA1/Serum Amyloid A) Polyclonal Antibody, Unconjugated (bs-19359R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining