bs-1910R [Primary Antibody]
TGF Beta R3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TGF Beta R3

Immunogen Range: 701-850/850


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7049

Swiss Prot: Q03167

Source: KLH conjugated synthetic peptide derived from human TGFBR3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Membrane Receptors Transforming growth factor beta is a multifunctional cytokine known to modulate several tissue development and repair processes, including cell differentiation, cell cycle progression, cellular migration, adhesion, and extracellular matrix production. There are 3 forms encoded by separate genes TGFB1, TGFB2, and TGFB3. The diverse effects of TGF beta are mediated by the TGF beta receptors and cell surface binding proteins. In addition to type I TGF beta receptor (TGFBR1) and type II (TFGBR2), type III (TGF beta III receptor) has been identified. It is a glycoprotein that binds TGF beta and exists in both a membrane bound and a soluble form. It may serve as a receptor accessory molecule in both the TGF beta and fibroblast growth factor systems. TGF beta III receptor lacks a recognizable signaling domain and has no clearly defined role in TGF beta signaling. Endothelial cells undergoing epithelial mesenchymal transformation express TGF beta III receptor, and TGF beta III receptor specific antisera inhibits mesenchyme formation and migration. Misexpression of TGF beta III receptor in nontransforming ventricular endothelial cells conferrs transformation in response to TGFB2. These results support a model where TGF beta III receptor localizes transformation in the heart and plays an essential, nonredundant role in TGF beta signaling. TGF beta III receptor, or beta glycan, can function as an inhibin coreceptor with ActRII. TGF beta III receptor binds inhibin with high affinity and enhances binding in cells coexpressing ActRII and TGF beta III receptor. Inhibin forms crosslinked complexes with both recombinant and endogenously expressed TGF beta III receptor and ActRII. TGF beta III receptor confers inhibin sensitivity to cell lines that otherwise respond poorly to this hormone.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 94


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Sheep
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chao Ye. et al. Naringin and bone marrow mesenchymal stem cells repair articular cartilage defects in rabbit knees through the transforming growth factor?β superfamily signaling pathway. Exp Ther Med. 2020 Nov;20(5):1-1Read more>>
  • Chao Ye. et al. Naringin in the repair of knee cartilage injury via the TGF-/ALK5/Smad2/3 signal transduction pathway combined with an acellular dermal matrix. J Orthop Transl. 2021 AugRead more>>
VALIDATION IMAGES

Lane 1: K562 Cells; Lane 2: HepG2 Cells; Lane 3: Hela Cells; Probed with TGF Beta R3 Polyclonal Antibody (bs-1910R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Placenta tissue lysates; Lane 4: Rat Testis tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human HeLa cell lysates; Lane 7: Human A431 cell lysates probed with TGF beta Receptor III Polyclonal Antibody, Unconjugated (bs-1910R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.