Host: Rabbit
Target Protein: Synphilin 1
Immunogen Range: 51-150/919
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9627
Swiss Prot: Q9Y6H5
Source: KLH conjugated synthetic peptide derived from human Synphilin 1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 100
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Guo Q et al. Small interfering RNA delivery to the neurons near the amyloid plaques for improved treatment of Alzheimer's disease. Acta Pharm Sin B. 2019 May;9(3):590-603.Read more>>
Rat brain lysates probed with Anti Synphilin-1 Polyclonal Antibody, Unconjugated (bs-1905R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 100kD. Observed band size:60kD 100kD.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Synphilin-1 Polyclonal Antibody, Unconjugated (bs-1905R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synphilin 1 Polyclonal Antibody, Unconjugated (bs-1905R) at 1:200 overnight at 4°C, DAB staining.