bs-19051R [Primary Antibody]
NCOA4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NCOA4

Immunogen Range: 1-100/614


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8031

Swiss Prot: Q13772

Source: KLH conjugated synthetic peptide derived from human NCOA4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()
ICC(1:100-500)

Predicted Molecular Weight: 70


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ke Xie. et al. Exosomes derived from M2-type microglia ameliorate oxygen-glucose deprivation/reoxygenation-induced HT22 cell injury by regulating miR-124-3p/NCOA4-mediated ferroptosis.. HELIYON. 2023 Jun;9(7):e17592-e17592Read more>>
  • Zhong Gaolong. et al. Copper Exposure Induced Chicken Hepatotoxicity: Involvement of Ferroptosis Mediated by Lipid Peroxidation, Ferritinophagy, and Inhibition of FSP1-CoQ10 and Nrf2/SLC7A11/GPX4 Axis. BIOL TRACE ELEM RES. 2023 Jul;:1-11Read more>>
  • Kun Li. et al. Oxidative medicine and cellular longevity the role and mechanism of NCOA4 in ferroptosis induced by intestinal ischemia reperfusion. INT IMMUNOPHARMACOL. 2024 May;133:112155Read more>>
  • Cuicui Zhuang. et al. Escherichia coli infection induces ferroptosis in bovine mammary epithelial cells by activating the Wnt/-catenin pathway-mediated mitophagy. MITOCHONDRION. 2024 Sep;78:101921Read more>>
  • Daoai Wu. et al. Autologous Platelet-Rich Gel Accelerates Diabetic Wound Healing Through Inhibition of Ferritinophagy. INT J LOW EXTR WOUND. ;()Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NCOA4 Polyclonal Antibody, Unconjugated (bs-19051R) at 1:200 overnight at 4°C, DAB staining.