Host: Rabbit
Target Protein: Reprimo
Immunogen Range: 41-109/109
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 56475
Swiss Prot: Q9NS64
Source: KLH conjugated synthetic peptide derived from human Reprimo
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 12
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Cow
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Buchegger, Kurt, et al. "Immunohistochemical expression of RPRM is associated with low expression of proliferation marker Ki67 in patients with breast cancer." Int J Clin Exp Med 9.2 (2016): 3025-3032.Read more>>
Formalin-fixed and paraffin-embedded : rat breast tissue labeled with Rabbit Anti-Reprimo Polyclonal Antibody (bs-1885R), Unconjugated 1:200 followed by conjugation to the secondary PE antibody and DABI staining
Formalin-fixed and paraffin-embedded : rat breast tissue labeled with Rabbit Anti-Reprimo Polyclonal Antibody (bs-1885R), Unconjugated 1:200 followed by conjugation to the secondary PE antibody
Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Reprimo) Polyclonal Antibody, Unconjugated (bs-1885R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.