Host: Rabbit
Target Protein: DUSP1/MKP1
Specificity: This antibody may interact with DUSP4/MKP2
Immunogen Range: 151-250/367
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1843
Swiss Prot: P28562
Source: KLH conjugated synthetic peptide derived from human DUSP1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 39
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Tiss, Ali, et al. "MAP kinase phosphatase DUSP1 is overexpressed in human obese and modulated by physical activity." American Journal of Physiology-Endocrinology and Metabolism (2014): ajpendo-00577.Read more>>
- Kato, Hiroyuki, et al. "Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis." Oncotarget (2016).Read more>>
- Kato, Hiroyuki, et al. "Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis." Oncotarget 7.2 (2016): 2009.Read more>>
- Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653Read more>>
- Sha J et al. Dexmedetomidine improves acute stress‐induced liver injury in rats by regulating MKP‐1, inhibiting NF‐κB pathway and cell apoptosis. J Cell Physiol. 2019 Aug;234(8):14068-14078.Read more>>
- Sha J et al. Dexmedetomidine attenuates lipopolysaccharide-induced liver oxidative stress and cell apoptosis in rats by increasing GSK-3β/MKP-1/Nrf2 pathway activity via the α2 adrenergic receptor. Toxicol Appl Pharmacol. 2019 Feb 1;364:144-152. Read more>>
- Meng-ke Fan. et al. Siglec-15 Promotes Tumor Progression in Osteosarcoma via DUSP1/MAPK Pathway. Front Oncol. 2021; 11: 710689Read more>>
Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-DUSP1/MKP-1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DUSP1/MKP1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Mouse Pancreas lysates probed with DUSP1/MKP1 Polyclonal Antibody, Unconjugated (bs1851R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.