bs-1840R
[Primary Antibody]
Lamin B1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Lamin B1
Immunogen Range: 361-460/586
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4001
Swiss Prot: P20700
Source: KLH conjugated synthetic peptide derived from human Lamin B1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 64
Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Jiang Z et al. The Essential Oils and Eucalyptol From Artemisia vulgaris L. Prevent Acetaminophen-Induced Liver Injury by Activating Nrf2-Keap1 and Enhancing APAP Clearance Through Non-Toxic Metabolic Pathway. Front Pharmacol. 2019 Jul 25;10:782. Read more>>
- Wang Z et al. Aronia melanocarpa Prevents Alcohol-Induced Chronic Liver Injury via Regulation of Nrf2 Signaling in C57BL/6 Mice. Oxid Med Cell Longev. 2020 Jan 8;2020:4054520.Read more>>
- Zhao Y et al. Effect of mitochondrial quality control on the lycopene antagonizing DEHP-induced mitophagy in spermatogenic cells. Food Funct. 2020 Jul 22;11(7):5815-5826.Read more>>
- Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919Read more>>
- Gi Ho Lee. et al. Impressic Acid Attenuates the Lipopolysaccharide-Induced Inflammatory Response by Activating the AMPK/GSK3/Nrf2 Axis in RAW264.7 Macrophages. Int J Mol Sci. 2021 Jan;22(2):762Read more>>
- Yuan Gao. et al. Lactobacillus plantarum Y44 alleviates oxidative stress by regulating gut microbiota and colonic barrier function in Balb/C mice with subcutaneous D-galactose injection. Food Funct. 2021 Jan;12(1):373-386Read more>>
- Feng Yanzhong. et al. Ulva prolifera Extract Alleviates Intestinal Oxidative Stress via Nrf2 Signaling in Weaned Piglets Challenged With Hydrogen Peroxide. Front Immunol. 2020 Oct;11:284Read more>>
- Lijin Guo. et al. Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Hosts Immune Response to Coccidiosis. Front Physiol. 2020; 11: 573676Read more>>
- Xiping Yang. et al. The piperine derivative HJ105 inhibits A1-42-induced neuroinflammation and oxidative damage via the Keap1-Nrf2-TXNIP axis. Phytomedicine. 2021 Apr;:153571Read more>>
- Chun Dai. et al. A novel UV-curable extravascular stent to prevent restenosis of venous grafts. Compos Part B-Eng. 2021 Nov;225:10926Read more>>
- Wang, Huihui. et al. Anti-inflammatory and Antioxidant Effects of Pyrroloquinoline Quinone in L-NAME-Induced Preeclampsia-Like Rat Model. 2021 Sep 2Read more>>
- Xiao-Lin Huang. et al. Zoledronic acid inhibits osteoclastogenesis and bone resorptive function by suppressing RANKL?mediated NF?B and JNK and their downstream signalling pathways. Mol Med Rep. 2022 Feb;25(2):1-12Read more>>
- Jinsheng Li. et al. Micro/nano-topography Promotes Osteogenic Differentiation of Bone Marrow Stem Cells by Regulating Periostin Expression. COLLOID SURFACE B. 2022 Jul;:1127Read more>>
- Pu, Luya. et al. Laminar shear stress alleviates monocyte adhesion and atherosclerosis development via miR-29b-3p/CX3CL1 axis regulation. J CELL SCI. 2022 Jul;135(14)Read more>>
- iqi Han. et al. Effects of thiacloprid exposure on microbiotaCgutCliver axis: Multiomics mechanistic analysis in Japanese quails. J HAZARD MATER. 2023 Jan;442:130082Read more>>
- Chi Li. et al. Schisantherin D from Schisandra chinensis (Turcz.) Baill. exhibits anti-liver fibrosis capacity via modulating ETBR involved signaling, an in vitro and in vivo study. FITOTERAPIA. 2022 Oct;162:10529Read more>>
- Jia-Xin Wang. et al. Heme-oxygenase-1 as a target for phthalate-induced cardiomyocytes ferroptosis. ENVIRON POLLUT. 2023 Jan;317:120717Read more>>
- Ruonan Wang. et al. The role of mitochondrial dynamics imbalance in hexavalent chromium-induced apoptosis and autophagy in rat testis. CHEM-BIOL INTERACT. 2023 Apr;374:110424Read more>>
- Junling Gao. et al. Mechanisms underlying the synergistic effects of chuanxiong combined with Chishao on treating acute lung injury based on network pharmacology and molecular docking combined with preclinical evaluation. J ETHNOPHARMACOL. 2024 May;325:117862Read more>>
VALIDATION IMAGES
Rat brain lysate probed with Anti Lamin B [Nuclear Envelope Marker] Polyclonal Antibody, Unconjugated (bs-1840R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 64kD. Observed band size: 64kD
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lamin B1 Polyclonal Antibody(bs-1840R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with lamin B1 polyclonal antibody, unconjugated (bs-1840R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.