Host: Rabbit
Target Protein: phospho-LIM Kinase 2 (Thr505)
Modification Site: Thr505
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3985
Swiss Prot: P53671
Source: KLH conjugated synthesised phosphopeptide derived from human LIM Kinase 1 around the phosphorylation site of Thr505
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
ELISA(ELISA=1:5000-10000)
IHC-P(IHC-P=1:100-500)
Predicted Molecular Weight: 70
Cross Reactive Species: Rat
Predicted Cross Reactive Species:
Human
Mouse
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
Zebrafish
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-LIM Kinase 2 (Thr505) ) Polyclonal Antibody, Unconjugated (bs-3247R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-LIM Kinase 2 (Thr505) ) Polyclonal Antibody, Unconjugated (bs-3247R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.