Host: Rabbit
Target Protein: IL-12
Immunogen Range: 51-150/219
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3592
Swiss Prot: P29459
Source: KLH conjugated synthetic peptide derived from human IL-12
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 23
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
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paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-IL12 Polyclonal Antibody, Unconjugated (bs-1789R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAPI staining
U251 lysates probed with IL12 Polyclonal Antibody, Unconjugated (bs-1789R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.