bs-1778R [Primary Antibody]
GATA4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GATA4

Specificity: May also interact with GATA-5, GATA-6

Immunogen Range: 261-360/442


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2626

Swiss Prot: P43694

Source: KLH conjugated synthetic peptide derived from human GATA-4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 49


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900Read more>>
  • Yang Z et al. A pilot study on polycystic ovarian syndrome caused by neonatal exposure to tributyltin and bisphenol A in rats. Chemosphere. 2019 Sep;231:151-160. Read more>>
  • Kim CW et al. Effects of cigarette smoke components on myocardial differentiation of mouse embryonic stem cells. Environ Toxicol. 2019 Sep 10.Read more>>
  • Wu P et al. Fluoride induces autoimmune orchitis involved with enhanced IL-17A secretion in mice testis. J Agric Food Chem. 2019 Dec 4;67(48):13333-13343.Read more>>
  • Sung-Moo Lee. et al. Effect of the phenylpyrrole fungicide fludioxonil on cell proliferation and cardiac differentiation in mouse embryonic stem cells. Reprod Toxicol. 2021 Sep;104:76Read more>>
  • Liu, Wen. et al. Transcriptome sequencing of LMP2A-transfected gastric cancer cells identifies potential biomarkers in EBV-associated gastric cancer. VIRUS GENES. 2022 Jul;:1-12Read more>>
  • Adam Mitchell. et al. Rapid Generation of Pulmonary Organoids from Induced Pluripotent Stem Cells by Co-Culturing Endodermal and Mesodermal Progenitors for Pulmonary Disease Modelling. BIOMEDICINES. 2023 May;11(5):1476Read more>>
  • Kexin Gao. et al. GATA4: Regulation of expression and functions in goat granulosa cells. DOMEST ANIM ENDOCRIN. 2024 Oct;89:106859Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human embryonic cells labeled with Anti-GATA-4, Unconjugated (bs-1778R) followed by conjugation to the secondary antibody and DAB staining


Lane 1: rat brain lysates Lane 2: rat liver lysates probed with Anti GATA4 Polyclonal Antibody, Unconjugated (bs-1778R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 49kD. Observed band size: 49kD.