bs-1777R [Primary Antibody]
Gastrin receptor/CCKBR Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Gastrin receptor/GASR/CCKBR

Immunogen Range: 301-400/447


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 887

Swiss Prot: P32239

Source: KLH conjugated synthetic peptide derived from human Gastrin receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mohammad, Shahid, et al. "Functional compensation between cholecystokinin-1 and-2 receptors in murine paraventricular nucleus neurons." Journal of Biological Chemistry 287.47 (2012): 39391-39401.Read more>>
  • Mohammad et al. Functional compensation between cholecystokinin-1 and -2 receptors in murine paraventricular nucleus neurons. (2012) J.Biol.Che. 287:39391-401Read more>>
  • Lu-Lu Duan. et al. Spinal CCK contributes to somatic hyperalgesia induced by orofacial inflammation combined with stress in adult female rats. Eur J Pharmacol. 2021 Dec;913:174619Read more>>
  • Ting Xiang. et al. Spinal CCK1 receptors contribute to somatic pain hypersensitivity induced by malocclusion via a reciprocal neuron-glial signaling cascade. J PAIN. 2022 JuRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Gastrin receptor Polyclonal Antibody, Unconjugated (bs-1777R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse Stomach lysates probed with CCKBR Polyclonal Antibody, Unconjugated (bs-1777R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates probed with Gastrin receptor/CCKBR Polyclonal Antibody, Unconjugated (bs-1777R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Gastrin receptor/CCKBR Polyclonal Antibody, Unconjugated (bs-1777R) at 1:200 overnight at 4°C, DAB staining.