DATASHEET
Host:
Rabbit
Target Protein:
METTL3
Immunogen Range:
2-100/580
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
56339
Swiss Prot:
Q86U44
Source:
KLH conjugated synthetic peptide derived from human METTL3
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation.
PRODUCT SPECIFIC PUBLICATIONS
- Wu F et al. Elevated Expression of Inhibitor of Apoptosis-stimulating Protein of p53 (iASPP) and Methyltransferase-like 3 (METTL3) Correlate with Poor Prognosis in FIGO Ib1-IIa Squamous Cell Cervical Cancer. J Cancer. 2020 Feb 10;11(9):2382-2389.Read more>>
- Wu F et al. Elevated Expression of Inhibitor of Apoptosis-stimulating Protein of p53 (iASPP) and Methyltransferase-like 3 (METTL3) Correlate with Poor Prognosis in FIGO Ib1-IIa Squamous Cell Cervical Cancer. J Cancer. 2020 Feb 10;11(9):2382-2389.Read more>>
- Tengjiao Qu. et al. Changes and relationship of N6-methyladenosine modification and long non-coding RNAs in oxidative damage induced by cadmium in pancreatic -cells. Toxicol Lett. 2021 Jun;343:56Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with METTL3 Polyclonal Antibody, Unconjugated (bs-17609R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with METTL3 Polyclonal Antibody, Unconjugated (bs-17609R) at 1:200 overnight at 4°C, DAB staining.