bs-17567R [Primary Antibody]
OTUD6B Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: OTUD6B

Immunogen Range: 51-150/293


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 51633

Swiss Prot: Q8N6M0

Source: KLH conjugated synthetic peptide derived from human OTUD6B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the ovarian tumor domain (OTU)-containing subfamily of deubiquitinating enzymes. Deubiquitinating enzymes are primarily involved in removing ubiquitin from proteins targeted for degradation. This protein may function as a negative regulator of the cell cycle in B cells. [provided by RefSeq, Nov 2013]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 34


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Dog
Rabbit
yeast

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhongqun Wang. et al. Loss of OTUD6B Stimulates Angiogenesis and Promotes Diabetic Atherosclerosis. DIABET METAB SYND OB. 2022 Sep;15:3027-3038Read more>>
  • injuan Ma. et al. MCTS1 enhances the proliferation of laryngeal squamous cell carcinoma via promoting OTUD6B-1 mediated LIN28B deubiquitination. BIOCHEM BIOPH RES CO. 2023 Oct;678:128Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with OTUD6B Polyclonal Antibody, Unconjugated (bs-17567R) at 1:400 overnight at 4°C, DAB staining.


Jurkat cell lysates probed with OTUD6B Polyclonal Antibody, Unconjugated (bs-17567R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.