bs-1744R [Primary Antibody]
CK7 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CK7

Immunogen Range: 251-350/469


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 110310

Swiss Prot: Q9DCV7

Source: KLH conjugated synthetic peptide derived from mouse CK7

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 54


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Ni-Ni, et al. "Functional regeneration of irradiated salivary glands with human amniotic epithelial cells transplantation." International Journal of Clinical and Experimental Pathology? 6.10 (2013): 2039-2047.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-CK7 Polyclonal Antibody, Unconjugated (bs-1744R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Mouse embryo lysates probed with Cytokeratin 7 Polyclonal Antibody, Unconjugated (bs-1744R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse large intestine lysates; Lane 2: Rat large intestine lysates probed with CK7 Polyclonal Antibody, Unconjugated (bs-1744R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cytokeratin 7) polyclonal Antibody, Unconjugated (bs-1744R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Polyclonal Antibody, Unconjugated (bs-1744R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Polyclonal Antibody, Unconjugated (bs-1744R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Mouse Urinary bladder tissue lysates; Lane 2: Mouse Breast tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Mouse trachea tissue lysates; Lane 5: Rat Urinary bladder tissue lysates; Lane 6: Rat Placenta tissue lysates; Lane 7: Human Hela cell lysates; Lane 8: Human HepG2 cell lysates; Lane 9: Human Siha cell lysates; Lane 10: Human Huvec cell lysates probed with CK7 Polyclonal Antibody, Unconjugated (bs-1744R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.