Host: Rabbit
Target Protein: CYP 3A4, 3A7, 3A5, and 3A9
Specificity: Will detect CYP 3A4, 3A7, 3A5, and 3A9
Immunogen Range: 18-120/503
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1576
Source: KLH conjugated synthetic peptide derived from human CYP3A4
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 55
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Ito, Junitsu, et al. ʺAscorbic acid reverses the prolonged anesthetic action of pentobarbital in Akr1a knockout mice.ʺ Life Sciences (2013).Read more>>
- "Sun B et al. The interleukin-17 G-197A polymorphism is associated with cyclosporine metabolism and transplant rejection in liver transplant recipients. Pharmacogenomics. 2019 Feb 25."Read more>>
- in Yang. et al. HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in Mice. INT J MOL SCI. 2023 Jan;24(6):5686Read more>>
Lane 1: rat liver lysates Lane 2: human colon carcinoma lysates probed with Anti CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 55kD. Observed band size: 55kD.\n
Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-CYP3A4 Polyclonal Antibody, Unconjugated (bs-1742R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated (bs-0295G-FITC) used at 1:200 dilution for 40 minutes
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CYP3A4) polyclonal Antibody, Unconjugated (bs-1742R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.