Host: Rabbit
Target Protein: CX3CR1
Immunogen Range: 151-250/355
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1524
Swiss Prot: P49238
Source: KLH conjugated synthetic peptide derived from human CX3CR1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
Predicted Molecular Weight: 39
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Sun et al. Inflammatory signals from photoreceptor modulate pathological retinal angiogenesis via c-Fos. (2017) J.Exp.Med. 214:1753-1767Read more>>
- Nakai et al. IL-17A induces heterogeneous macrophages, and it does not alter the effects of lipopolysaccharides on macrophage activation in the skin of mice. (2017) Sci.Rep. 7:12473Read more>>
- Venosa Alessandro. et al. Role of CCR2+ Myeloid Cells in Inflammation Responses Driven by Expression of a Surfactant Protein-C Mutant in the Alveolar Epithelium. Front Immunol. 2021 Apr;12:1348Read more>>
- Takei, Yoshinori. et al. Alteration in peritoneal cells with the chemokine CX3CL1 reverses age-associated impairment of recognition memory. GEROSCIENCE. 2022 May;:1-14Read more>>
- Tianrui Zhang. et al. Daphnetin Improves Neuropathic Pain by Inhibiting the Expression of Chemokines and Inflammatory Factors in the Spinal Cord and Interfering with Glial Cell Polarization. PHARMACEUTICALS-BASE. 2023 Feb;16(2):243Read more>>
- Lu Wang. et al. Dysfunctional synaptic pruning by microglia correlates with cognitive impairment in sleep-deprived mice: Involvement of CX3CR1 signaling. NEUROBIOL STRESS. 2023 Jul;25:100553Read more>>
- Wei Qin. et al. Molecular subtypes of lung adenocarcinoma patients for prognosis and therapeutic response prediction with machine learning on 13 programmed cell death patterns. J CANCER RES CLIN. 2023 Jun;:1-18Read more>>
- Rondeaux Julie. et al. Ezh2 emerges as an epigenetic checkpoint regulator during monocyte differentiation limiting cardiac dysfunction post-MI. NAT COMMUN. 2023 Jul;14(1):1-15Read more>>
- Duliurui Huang. et al. Analysis of the heterogeneity and complexity of murine extraorbital lacrimal gland via single-cell RNA sequencing. OCUL SURF. 2024 JuRead more>>
Mouse splenocytes probed with Rabbit Anti-CX3CR1 Polyclonal Antibody, FITC Conjugated (bs-1728R-FITC) (green) at 1:100 for 40 minutes compared to control cells (blue).\n
Lane 1: rat brain lysates Lane 2: mouse heart lysates probed with Anti CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 39kD. Observed band size: 39kD.
Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse Spleen cell lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CX3CR1 Polyclonal Antibody(bs-1728R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CX3CR1) Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Spinal cord tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with CX3CR1 Polyclonal Antibody, Unconjugated (bs-1728R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.