DATASHEET
Host:
Rabbit
Target Protein:
Cytokeratin 13
Immunogen Range:
251-350/458
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
3860
Source:
KLH conjugated synthetic peptide derived from human Cytokeratin-13
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008].
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti- Cytokeratin 13 Antibody, conjugated (bs-1717R-AF488) at 1:200 overnight at 4°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
Lane 1: Mouse Uterus tissue lysates; Lane 2: Mouse Urinary bladder tissue lysates; Lane 3: Mouse Ear tissue lysates probed with Cytokeratin 13 Polyclonal Antibody, Unconjugated (bs-1717R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.