Host: Rabbit
Target Protein: DAT/Dopamine transporter
Immunogen Range: 579-620/620
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6531
Swiss Prot: Q01959
Source: KLH conjugated synthetic peptide derived from human DAT1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 68
Cross Reactive Species:
Human
Mouse
Rat
Others
Predicted Cross Reactive Species:
Dog
Cow
For research use only. Not intended for diagnostic or therapeutic use.
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- Romei, Cristina, et al. "Colocalization of neurotransmitter transporters on the plasma membrane of the same nerve terminal may reflect cotransmission."Brain Research Bulletin (2016).Read more>>
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- ai L et al. m6A demethylase FTO regulates dopaminergic neurotransmission deficits caused by arsenite.Toxicol Sci. 2018 Oct 1;165(2):431-446.Read more>>
- Shi L et al. Clioquinol improves motor and non-motor deficits in MPTP-induced monkey model of Parkinson's disease through AKT/mTOR pathway. Aging (Albany NY). 2020 May 18;12(10):9515-9533.Read more>>
- Maria Teresa Rivera-Garciaet al. Sex and strain differences in dynamic and static properties of the mesolimbic dopamine system. Neuropsychopharmacology. 2020 Nov;45(12):2079-2086.Read more>>
- Chenyu Zhang. et al. The Nrf2-NLRP3-caspase-1 axis mediates the neuroprotective effects of Celastrol in Parkinson's disease. Redox Biol. 2021 Nov;47:102134Read more>>
- Huang Jian. et al. Icariside II Attenuates Methamphetamine-Induced Neurotoxicity and Behavioral Impairments via Activating the Keap1-Nrf2 Pathway. Oxid Med Cell Longev. 2022;2022:8400876Read more>>
- Paul C. Omaliko. et al. Impact of Dietary Fat Types on expression levels of Dopamine and Serotonin transporters in the Ileum of broiler chickens. POULTRY SCI. 2024 Jul;:104114Read more>>
RSC96 cells probed with Rabbit Anti-DAT/Dopamine transporter Polyclonal Antibody (bs-1714R ) at 6ug for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Dopamine Transporter Polyclonal Antibody, Unconjugated (bs-1714R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Rat Cerebrum lysates probed with Dopamine Transporter Polyclonal Antibody, Unconjugated (bs-1714R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DAT/Dopamine transporter Polyclonal Antibody, Unconjugated (bs-1714R) at 1:400 overnight at 4°C, DAB staining.
RSC96 cells probed with DAT/Dopamine transporter Polyclonal Antibody, PE-Conjugated (bs-1714R-PE; green) at 1:33 for 30 minutes, compared to isotype control (bs-0295P-PE; orange) and non-stained cells (blue).