bs-1712R
[Primary Antibody]
pan Cytokeratin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: pan Cytokeratin
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated synthetic peptide derived from human pan cytokeratin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes.
In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 42-64
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Cow
Predicted Cross Reactive Species:
Dog
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Chen, Ming, et al. "Periostin activates pathways involved in epithelial-mesenchymal transition in adamantinomatous craniopharyngioma." Journal of the Neurological Sciences (2015).Read more>>
- Cheng, Pang, et al. "Colocalization of Metastasis-associated proteins 1/2 and Estrogen receptor alpha in rat epididymis." Tissue and Cell (2017).Read more>>
- Li, Xin, et al. "Enrichment of Oct3/4‐positive cells from a human bronchial epithelial cell line." Apmis 121.7 (2013): 612-625.Read more>>
- Yang, Wei, et al. "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." PeerJ 5 (2017): e3950.Read more>>
- Wang et al. Anti-proliferative activities of finasteride in benign prostate epithelial cells require stromal fibroblasts and c-Jun gene. (2017) PLoS.One. 12:e0172233Read more>>
- Poruk et al. Circulating Tumor Cells Expressing Markers of Tumor-Initiating Cells Predict Poor Survival and Cancer Recurrence in Patients with Pancreatic Ductal Adenocarcinoma. (2017) Clin.Cancer.Res. 23:2681-2690Read more>>
- echbuhl HM et al. Fibroblast subtypes define a metastatic matrisome in breast cancer. JCI Insight. 2020 Feb 27;5(4). Read more>>
- Mahmoud Labibet al. Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Nat Biomed Eng. 2020 Jul 27.Read more>>
- Wakiyama H et al. Increased Immunogenicity of a Minimally Immunogenic Tumor after Cancer-Targeting Near Infrared PhotoimmunotherapyCancers (Basel).2020 Dec 12;12(12):3747.Read more>>
- Yasuhiro Maruoka. et al. Interleukin-15 after Near-Infrared Photoimmunotherapy (NIR-PIT) Enhances T Cell Response against Syngeneic Mouse Tumors. Cancers. 2020 Sep;12(9):2575Read more>>
- Yasuhiro Maruoka. et al. Near-Infrared Photoimmunotherapy Combined with CTLA4 Checkpoint Blockade in Syngeneic Mouse Cancer Models. Vaccines-Basel. 2020 Sep;8(3):528Read more>>
- Ryuhei Okada. et al. Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials. Ebiomedicine. 2021 May;67:103345Read more>>
- Hui Ren. et al. Arecoline induces epithelial\mesenchymal transformation and promotes metastasis of oral cancer by SAA1 expression. Cancer Sci. 2021 Jun; 112(6): 2173C2184Read more>>
- Verdiana Trappetti. et al. Synchrotron Microbeam Radiotherapy for the treatment of lung carcinoma: a pre-clinical study. Int J Radiat Oncol. 2021 AugRead more>>
- Tomchaney, M.. et al. Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease. Bmc Pulm Med. 2021 Dec;21(1):1-14Read more>>
- Chuanli Zhang. et al. Establishment of a human meibomian gland carcinoma cell model and analysis of differently expressed genes. Exp Eye Res. 2022 Feb;:108983Read more>>
- Yan Wu. et al. A mesenchymal stem cell-derived nanovesicle-biopotentiated bovine serum albumin-bridged gelatin hydrogel for enhanced diabetic wound therapy. MATER DESIGN. 2023 Jun;230:11196Read more>>
- Chen Mingyu. et al. Identification of RAC1 in promoting brain metastasis of lung adenocarcinoma using single-cell transcriptome sequencing. CELL DEATH DIS. 2023 May;14(5):1-14Read more>>
- Denise Bonente. et al. Co-Expression of Podoplanin and CD44 in Proliferative Vitreoretinopathy Epiretinal Membranes. INT J MOL SCI. 2023 Jan;24(11):9728Read more>>
- Fukushima Hiroshi. et al. Intratumoral interleukin-15 improves efficacy of near-infrared photoimmunotherapy. MOL CANCER THER. 2023 JulRead more>>
- Jian Shi. et al. Mechanistic elucidation of QiJu-DiHuang Wan in management of age-related dry eye through metabolomics and network pharmacology. PHYTOMEDICINE. 2024 Sep;132:155884Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human liver carcinoma tissue labeled with Anti-P-CK/Cytokeratin AE1+AE3 Polyclonal Antibody, Unconjugated (bs-1712R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:400 overnight at 4°C, DAB staining.
Mouse Bladder lysates probed with Pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human A673 cell lysates probed with pan Cytokeratin Polyclonal Antibody, Unconjugated (bs-1712R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Pan Cytokeratin) polyclonal Antibody, Unconjugated (bs-1712R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with pan Cytokeratin Polyclonal Antibody(bs-1712R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (bs-1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (bs-1712R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (bs-1712R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Pan Cytokeratin Polyclonal Antibody(bs-1712R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: pan Cytokeratin Polyclonal Antibody, conjugated (bs-1712R –AF555) at 1:200 overnight at 4°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.