DATASHEET
Host:
Rabbit
Target Protein:
Wnt3a
Immunogen Range:
281-352/352
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
89780
Swiss Prot:
P56704
Source:
KLH conjugated synthetic peptide derived from human Wnt3a
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
PRODUCT SPECIFIC PUBLICATIONS
- Gao, Peng, et al. "Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling." Molecular Medicine Reports.Read more>>
- Lu, Juan, et al. "Expression of Wnt3a, Wnt10b, β-catenin and DKK1 in periodontium during orthodontic tooth movement in rats." Acta Odontologica Scandinavica (2015): 1-7.Read more>>
- Hayashi, Kouhei, et al. "Involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland." Biochemical and Biophysical Research Communications (2016).Read more>>
- Böscke, Robert, et al. "Wnt Signaling in Chronic Rhinosinusitis with Nasal Polyps." American Journal of Respiratory Cell and Molecular Biology ja (2017).Read more>>
- Zhu, Afang, et al. "Suppression of Wnt5a, but not Wnts, relieves chronic post-thoracotomy pain via anti-inflammatory modulation in rats." Biochemical and Biophysical Research Communications (2017).Read more>>
- Jin, Xinhao, et al. "Environmental Enrichment Improves Spatial Learning and Memory in Vascular Dementia Rats with Activation of Wnt/β-Catenin Signal Pathway." Medical science monitor: international medical journal of experimental and clinical research 23 (2017): 207.Read more>>
- Guo, Furong, et al. "Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease." Experimental and Therapeutic Medicine.(2017)Read more>>
- Guo et al. Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease. (2017) Exp.Ther.Me. 14:4231-4237Read more>>
- Sun et al. miR-491-5p, mediated by Foxi1, functions as a tumor suppressor by targeting Wnt3a/β-catenin signaling in the development of gastric cancer. (2017) Cell.Death.Dis. 8:e2714Read more>>
- Yang et al. MicroRNA-195 acts as a tumor suppressor by directly targeting Wnt3a in HepG2 hepatocellular carcinoma cells. (2014) Mol.Med.Rep. 10:2643-8Read more>>
- Xu T et al. MicroRNA\708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.Read more>>
- Yang Fan. et al. Catalpol Protects Against Pulmonary Fibrosis Through Inhibiting TGF-1/Smad3 and Wnt/-Catenin Signaling Pathways. Front Pharmacol. 2021 Jan;11:2472Read more>>
- Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094Read more>>
- Yanyan Xing. et al. Effects of mechanosensitive ion channel Piezo1 on proliferation and osteogenic differentiation of human dental follicle cells. Ann Anat. 2021 Oct;:151847Read more>>
- Huan He. et al. BDE-209 disturbed proliferation and differentiation of spermatogonia during mitotic process through estrogen receptor . REPROD BIOL. 2023 Jun;23:100737Read more>>
- Cai Zhijun. et al. Overloaded axial stress activates the Wnt/-Catenin pathway in nucleus pulposus cells of adult degenerative scoliosis combined with intervertebral disc degeneration. MOL BIOL REP. 2023 Apr;:1-8Read more>>
- Ippei Horibe. et al. Acquired curved hair is caused by fusion of multiple hair matrix cells. J DERMATOL SCI. 2024 FebRead more>>
- Nobuhiro Nakazawa. et al. Poorly cohesive gastric cancer with increased epithelialmesenchymal transition is associated with a poor prognosis. ONCOL LETT. 2024 Sep;28(3):1-8Read more>>
VALIDATION IMAGES
L1 rat brain, L2 mouse uterus lysates probed (bs-1700R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 37kDa.
Formalin-fixed and paraffin embedded rat ovary tissue labeled with Anti-Wnt3a Polyclonal Antibody (bs-1700R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Lane 1: U2os cell lysates probed with Wnt3a Polyclonal Antibody, Unconjugated (bs-1700R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.