bs-1698R
[Primary Antibody]
ox-LDL Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ox-LDL
Clonality: Polyclonal
Isotype: IgG
Source: Full length plasma protein (Human)
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 31
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wen, Song, et al. "OxLDL-targeted iron oxide nanoparticles for in vivo MRI detection of perivascular carotid collar induced atherosclerotic lesions in ApoE-deficient mice." Journal of Lipid Research 53.5 (2012): 829-838.Read more>>
- Martinez, Jennifer, et al. "Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins." Nature Cell Biology (2015).Read more>>
- Wang et al. Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy. (2016) Int.J.Clin.Exp.Patho. 8:15696-707Read more>>
- Xia Zhanget al. NEAT1 knockdown suppresses endothelial cell proliferation and induces apoptosis by regulating miR?638/AKT/mTOR signaling in atherosclerosis. Oncol Rep. 2020 Jul;44(1):115-125.Read more>>
- Song, Bangrong. et al. Differential Expression of LOXL1-AS1 in Coronary Heart Disease and its Regulatory Mechanism in ox-LDL-Induced Human Coronary Artery Endothelial Cell Pyroptosis. 2021 Oct 11Read more>>
- Fen Gao. et al. Angiotensin II induces the expression of periostin to promote foam cell formation in oxLDL-treated macrophages. Int J Cardiol. 2022 Jan;347:46Read more>>
- Wang, Xin-Yuan. et al. Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study. Lipids Health Dis. 2021 Dec;20(1):1-13Read more>>
- Takuya Tsumita. et al. The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells. INT J CANCER. 2022 May 24Read more>>
- Haikuo Li. et al. Comprehensive single-cell transcriptional profiling defines shared and unique epithelial injury responses during kidney fibrosis. CELL METAB. 2022 OcRead more>>
- Qing Li. et al. Re-Visiting Antioxidant Therapy in Murine Advanced Atherosclerosis with Brussels Chicory, a Typical Vegetable in Mediterranean Diets. NUTRIENTS. 2023 Jan;15(4):832Read more>>
- Liu Chan-Jung. et al. Increased renal uptake and urine excretion of oxidized LDL is possibly associated with formation of large calcium oxalate nephrolithiasis: a preliminary study. WORLD J UROL. 2023 Mar;:1-8Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human colorectal cancer labeled with Anti-oxLDL Polyclonal Antibody, Unconjugated (bs-1698R) followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ox-LDL Polyclonal Antibody, Unconjugated (bs-1698R) at 1:400 overnight at 4°C, DAB staining.
A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ox-LDL Antibody（bs-1698R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
HepG2 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ox-LDL Polyclonal Antibody(bs-1698R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).