Host: Rabbit
Target Protein: MEK3/MAP2K3
Immunogen Range: 251-347/347
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5606
Swiss Prot: P46734
Source: KLH conjugated synthetic peptide derived from human MKK3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 39
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Li Meng. et al. Comprehensive Analysis of 5-Methylcytosine Profiles of Messenger RNA in Human High-Grade Serous Ovarian Cancer by MeRIP Sequencing. Cancer Manag Res. 2021 Aug;13:6005-6018Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Anti-MEK3/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MEK3/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: rat skeletal muscle lysates probed with MEK3/MAP2K3 Polyclonal Antibody, Unconjugated (bs-1689R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.