DATASHEET
Host:
Rabbit
Target Protein:
LACTB
Immunogen Range:
101-200/547
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
114294
Swiss Prot:
P83111
Source:
KLH conjugated synthetic peptide derived from human LACTB
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
This gene encodes a mitochondrially-localized protein that has sequence similarity to prokaryotic beta-lactamases. Many of the residues responsible for beta-lactamase activity are not conserved in this protein, suggesting it may have a different enzymatic function. Increased expression of the related mouse gene was found to be associated with obesity. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Dec 2013]
PRODUCT SPECIFIC PUBLICATIONS
- Yongrui Hai. et al. A novel mitochondria-targeting DHODH inhibitor induces robust ferroptosis and alleviates immune suppression. PHARMACOL RES. 2024 Apr;202:107115Read more>>
VALIDATION IMAGES
Mouse liver/kidney lysates probed with Anti-LACTB Polyclonal Antibody (bs-16882R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (LACTB ) Polyclonal Antibody, Unconjugated (bs-16882R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.