bs-16681R [Primary Antibody]
phospho-HEF1 (Ser369) Polyclonal Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: HEF1

Modification Site: Ser369

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4739

Swiss Prot: Q14511

Source: KLH conjugated synthesised phosphopeptide derived from human HEF1 around the phosphorylation site of Ser369

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 93


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human A549 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human HepG2 cell lysates probed with phospho-HEF1 (Ser369) Polyclonal Antibody, Unconjugated (bs-16681R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.