bs-1658R
[Primary Antibody]
STAT3 (Tyr705) Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: STAT3 Tyr705
Modification Site: Tyr705
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6774
Swiss Prot: P40763
Source: KLH conjugated synthetic phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 85
Cross Reactive Species:
Human
Mouse
Rat
Pig
Chicken
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Lee E-J, Lee SJ, Kim J-H, Kim K-J, Yang S-H, Jeong K-Y, et al. (2016) Radiation Inhibits Interleukin-12 Production via Inhibition of C-Rel through the Interleukin-6/ Signal Transducer and Activator of Transcription 3 Signaling Pathway in Dendritic Cells. PLoS ONE 11(1): e0146463.Read more>>
- Sur, Swastika, et al. "Increased Expression of Phosphorylated Polo-Like Kinase 1 and Histone in Bypass Vein Graft and Coronary Arteries following Angioplasty." PloS one 11.1 (2016): e0147937.Read more>>
- Iriyama, Noriyoshi, Yoshihiro Hatta, and Masami Takei. "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." Cancer Medicine (2016).Read more>>
- Gao, Ling, et al. "Morusin shows potent antitumor activity for human hepatocellular carcinoma in vitro and in vivo through apoptosis induction and angiogenesis inhibition." Drug Design, Development and Therapy 11 (2017): 1789.Read more>>
- Zhang, Yu, et al. "JAK-STAT signaling regulation of chicken embryonic stem cell differentiation into male germ cells." In Vitro Cellular & Developmental Biology-Animal (2017): 1-16.Read more>>
- Badin et al. Muscle Arnt/Hif1β Is Dispensable in Myofiber Type Determination, Vascularization and Insulin Sensitivity. (2016) PLoS.One. 11:e0168457Read more>>
- Iriyama et al. Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes. (2016) Cancer.Med. 5:3223-3234Read more>>
- Wang G et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blockingthe TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev. 2019 Apr 28;2019:7850324.Read more>>
- Zhou H et al. Paris saponin VII extracted from trillium tschonoskii suppresses proliferation and induces apoptosis of human colorectal cancer cells. J Ethnopharmacol. 2019 Jul 15;239:111903. Read more>>
- Li B et al. Increased hepcidin in hemorrhagic plaques correlates with iron-stimulated IL-6/STAT3 pathway activation in macrophages. Biochem Biophys Res Commun. 2019 Jul 23;515(2):394-400. Read more>>
- Gao M et al. Activating the interleukin-6-Gp130-STAT3 pathway ameliorates ventricular electrical stability in myocardial infarction rats by modulating neurotransmitters in the paraventricular nucleus. BMC Cardiovasc Disord. 2020 Feb 5;20(1):60.Read more>>
- Hao Y et al. IL-6/STAT3 mediates the HPV18 E6/E7 stimulated upregulation of MALAT1 gene in cervical cancer HeLa cells. Virus Res. 2020 May;281:197907.Read more>>
- Gao M et al. Activating the interleukin-6-Gp130-STAT3 pathway ameliorates ventricular electrical stability in myocardial infarction rats by modulating neurotransmitters in the paraventricular nucleus. BMC Cardiovasc Disord. 2020 Feb 5;20(1):60.Read more>>
- Mengmeng Zhang. et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachlorideCinduced liver fibrosis in mice. N-S Arch Pharmacol. 2021 Jan;394(1):189-204Read more>>
- Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
- Xuejiao Zhou. et al. The novel ALK inhibitor ZX\29 induces apoptosis through inhibiting ALK and inducing ROS\mediated endoplasmic reticulum stress in Karpas299 cells. 2020 Nov 02Read more>>
- Yusi Liu. et al. Synergistic Effects of Resveratrol and Temozolomide Against Glioblastoma Cells: Underlying Mechanism and Therapeutic Implications. Cancer Manag Res. 2020; 12: 8341C8354Read more>>
- Jiajia Wang. et al. TAK-242 ameliorates DSS-induced colitis by regulating the gut microbiota and the JAK2/STAT3 signaling pathway. Microb Cell Fact. 2020 Dec;19(1):1-17Read more>>
- Yuying Li. et al. Anthraquinone derivative C10 inhibits proliferation and cell cycle progression in colon cancer cells via the Jak2/Stat3 signaling pathway. Toxicol Appl Pharm. 2021 Mar;:115481Read more>>
- Ai Jin. et al. TSLP-induced collagen type-I synthesis through STAT3 and PRMT1 is sensitive to calcitriol in human lung fibroblasts. Bba-Mol Cell Res. 2021 Jun;:119083Read more>>
- Yan Zhang. et al. Ganoderic Acid A To Alleviate Neuroinflammation of Alzheimers Disease in Mice by Regulating the Imbalance of the Th17/Tregs Axis. J Agr Food Chem. 2021;69(47):14204C14214Read more>>
- Hongwu Meng. et al. Interleukin-9 attenuates inflammatory response and hepatocyte apoptosis in alcoholic liver injury. Life Sci. 2022 Jan;288:12018Read more>>
- Leiming Sun. et al. Isoliquiritigenin attenuates acute renal injury through suppressing oxidative stress, fibrosis and JAK2/STAT3 pathway in streptozotocin-induced diabetic rats. Bioengineered. 2021;12(2):11188-112Read more>>
- Zong-Ding Wang. et al. A Novel Hepatectomy Model in Mice Using a Gutta Cutter Tool: A Feasibility Study and Preliminary Results. Transpl P. 2022 MaRead more>>
- Keliang Song. et al. Knocking down Siglec-15 in osteosarcoma cells inhibits proliferation while promoting apoptosis and pyroptosis by targeting the Siglec-15/STAT3/Bcl-2 pathway. ADV MED SCI-POLAND. Adv Med Sci-Poland. 2022 Sep;67:187Read more>>
- Feng Junlong. et al. Explore the Effect of Asthma Regulating HIF-1 Pathway on Sperm Quality Based on Rat Model. BIOMED RES INT. 2022;2022:4194685Read more>>
- Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17Read more>>
- Qin Yang. et al. Anti-tumor effect of infant-derived Enterococcus via the inhibition of proliferation and inflammation as well as the promotion of apoptosis. FOOD FUNCT. 2023 JaRead more>>
- Huang Yongpan. et al. Oxymatrine ameliorates myocardial injury by inhibiting oxidative stress and apoptosis via the Nrf2/HO-1 and JAK/STAT pathways in type 2 diabetic rats. BMC Complementary Medicine and Therapies. 2023 Dec;23(1):1-13Read more>>
- Li Xiangdong. et al. Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3. J TRANSL MED. 2023 Dec;21(1):1-19Read more>>
- Yong Wang. et al. An integrated network pharmacology approach reveals that Darutigenol reduces inflammation and cartilage degradation in a mouse collagen-induced arthritis model by inhibiting the JAK-STAT3 pathway. J ETHNOPHARMACOL. 2023 May;:116574Read more>>
- Yiran Chen. et al. Qinzhu Liangxue inhibits IL-6-induced hyperproliferation and inflammation in HaCaT cells by regulating METTL14/SOCS3/STAT3 axis. J ETHNOPHARMACOL. 2023 Dec;317:116809Read more>>
- Zhijie Wang. et al. Discovery of a Bromodomain and Extra Terminal Domain (BET) Inhibitor with the Selectivity for the Second Bromodomain (BD2) and the Capacity for the Treatment of Inflammatory Diseases. J MED CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
- Yuan Hongyu. et al. Hypoxia-induced TMTC3 expression in esophageal squamous cell carcinoma potentiates tumor angiogenesis through Rho GTPase/STAT3/VEGFA pathway. J EXP CLIN CANC RES. 2023 Dec;42(1):1-17Read more>>
- Wei Guo. et al. CircRNA-5335 Regulates the Differentiation and Proliferation of Sheep Preadipocyte via the miR-125a-3p/STAT3 Pathway. VET SCI. 2024 Feb;11(2):7Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-phospho-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human stomach cancer tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
293T cells probed with STAT3 (Tyr705) Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-1658R-A647) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).
Lane 1: Kindey lysates probed with STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brains; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Heart lysates; Lane 2: Mouse Kidney lysates; Lane 3: Rat Aorta lysates probed with STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-1658R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with STAT3 (Tyr705) Polyclonal Antibody(bs-1658R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-STAT3 (Tyr705) Polyclonal Antibody(bs-1658R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
25 ug total protein per lane of various lysates (see on figure) probed with phospho-STAT3 (Tyr705) polyclonal antibody, unconjugated (bs-1658R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.